Splicing isoform of SYT-SSX fusion protein accelerates transcriptional activity and cell proliferation

Yuki Morimoto, Mamoru Ouchida, Toshifumi Ozaki, Akira Kawai, Tatsuo Ito, Aki Yoshida, Hajime Inoue, Kenji Shimizu

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The human SYT-SSX gene has two splicing isoforms (type N and I), the latter of which contains an additional insertion of 93 bases. In the present study, we found increased transcriptional activity of the SYT-SSX type I protein in luciferase assay. When the SYT-SSX cDNAs were transfected to NIH3T3 cells, the type I transformant grew faster than the type N transformant. Furthermore, we evaluated the isoform ratio of the SYT or SYT-SSX transcripts in various tissues. Our results suggest that the SYT-SSX type I protein plays a critical role in the tumorigenesis of synovial sarcomas through increased transcriptional activity.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalCancer Letters
Volume199
Issue number1
DOIs
Publication statusPublished - Sep 10 2003

Keywords

  • Alternative splicing
  • Cell growth
  • SSX
  • SYT
  • Synovial sarcoma
  • Transcriptional activity

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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