Spiral trajectory modulation of rheotaxic motile human sperm in cylindrical microfluidic channels of different inner diameters

Saori Nishina, Koji Matsuura, Keiji Naruse

Research output: Contribution to journalArticle

Abstract

We investigated the relationship between human sperm rheotaxis and motile sperm trajectories by using poly- (dimethylsiloxane) (PDMS)-based cylindrical microfluidic channels with inner diameters of 100 μm, 50 μm, and 70 μm, which corresponded to the inner diameter of the human isthmus, the length of a sperm and a diameter intermediate between the two, respectively. We counted the number of rheotaxic sperm and sperm with spiral motion. We also analyzed motile sperm trajectories. As the cylindrical channel diameter was decreased, the percentage of sperm cells exhibiting rheotaxis, the percentage of sperm cells exhibiting spiral motion, the frequency-to-diameter ratio of the sperm cells' spiral trajectories, and the surface area of the microfluidic channel increased, while the flagellar motion at the channel wall decreased. The percentage of sperm exhibiting a spiral trajectory and the frequency-to-diameter ratio of the sperm cells' spiral trajectories were thus affected by the channel diameter. Our findings suggest that the oviduct structure affects the swimming properties of sperm cells, guiding them from the uterus to the ampulla for egg fertilization. These results could contribute to the development of motile sperm-sorting microfluidic devices for assisted reproductive technologies.

Original languageEnglish
Pages (from-to)213-221
Number of pages9
JournalActa medica Okayama
Volume73
Issue number3
Publication statusPublished - Jan 1 2019

Fingerprint

Microfluidics
Spermatozoa
Trajectories
Modulation
Sorting
Lab-On-A-Chip Devices
Assisted Reproductive Techniques
Sperm Count
Oviducts
Fertilization
Uterus
Ovum

Keywords

  • Microfluidic channel
  • Oviduct structure
  • Rheotaxis
  • Sperm motility
  • Trajectory

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Spiral trajectory modulation of rheotaxic motile human sperm in cylindrical microfluidic channels of different inner diameters. / Nishina, Saori; Matsuura, Koji; Naruse, Keiji.

In: Acta medica Okayama, Vol. 73, No. 3, 01.01.2019, p. 213-221.

Research output: Contribution to journalArticle

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