Spinal high-mobility group box-1 induces long-lasting mechanical hypersensitivity through the toll-like receptor 4 and upregulation of interleukin-1β in activated astrocytes

Norimitsu Morioka, Kazuki Miyauchi, Keita Miyashita, Takahiro Kochi, Fang Fang Zhang, Yoki Nakamura, Keyue Liu, Hidenori Wake, Kazue Hisaoka-Nakashima, Masahiro Nishibori, Yoshihiro Nakata

Research output: Contribution to journalArticle

Abstract

Intrathecal treatment with recombinant high-mobility group box-1 (rHMGB1) in naïve mice leads to a persistent and significantly decreased hind paw withdrawal threshold to mechanical stimuli, suggesting that spinal HMGB1 evokes abnormal pain processing. By contrast, repeated intrathecal treatment with anti-HMGB1 antibody significantly reverses hind paw mechano-hypersensitivity in mice with a partial sciatic nerve ligation (PSNL). By contrast, the cellular mechanism by which spinal HMGB1 induces neuropathic pain has yet to be fully elaborated. The current study tested the hypothesis that spinal HMGB1 could induce mechanical hypersensitivity through the activation of specific receptor in glial cells. Intrathecal pretreatment with toll-like receptor (TLR) 4 inhibitors, but not TLR5, receptor for advanced glycation end-products and C-X-C chemokine receptor type 4 inhibitors, prevented rHMGB1-evoked mechanical hypersensitivity. Activation of spinal astrocytes appears to be crucial for the mechanism of action of rHMGB1 in naïve mice, as intrathecal pretreatment with astrocytic inhibitors prevented the rHMGB1-induced mechanical hypersensitivity. Interleukin-1β (IL-1β) was up-regulated within activated astrocytes and block of TLR4 prevented the upregulation of IL-1β. Interleukin-1β appears to be secreted by activated astrocytes, as IL-1β neutralizing antibody prevented rHMGB1-induced mechanical hypersensitivity. Furthermore, intrathecal pretreatment with either MK801 or gabapentin prevented the rHMGB1-induced mechanical hypersensitivity, suggesting roles for spinal glutamate and the N-methyl-d-aspartate receptor in the mediation of rHMGB1-induced mechanical hypersensitivity. Thus, the current findings suggest that spinal HMGB1 upregulates IL-1β in spinal astrocytes through a TLR4-dependent pathway and increases glutamatergic nociceptive transduction. These spinal mechanisms could be key steps that maintain neuropathic pain. (Figure presented.).

Original languageEnglish
Pages (from-to)738-758
Number of pages21
JournalJournal of Neurochemistry
Volume150
Issue number6
DOIs
Publication statusPublished - Sep 1 2019

Fingerprint

HMGB1 Protein
Toll-Like Receptor 4
Interleukin-1
Astrocytes
Hypersensitivity
Up-Regulation
Toll-Like Receptor 5
Neuralgia
Chemical activation
CXC Chemokines
Chemokine Receptors
Neutralizing Antibodies
Glutamic Acid
Sciatic Nerve
Neuroglia
Ligation
Anti-Idiotypic Antibodies
Antibodies
Processing
Pain

Keywords

  • allodynia
  • glutamate
  • HMGB1
  • interleukin-1β
  • neuropathic pain
  • TLR4

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Spinal high-mobility group box-1 induces long-lasting mechanical hypersensitivity through the toll-like receptor 4 and upregulation of interleukin-1β in activated astrocytes. / Morioka, Norimitsu; Miyauchi, Kazuki; Miyashita, Keita; Kochi, Takahiro; Zhang, Fang Fang; Nakamura, Yoki; Liu, Keyue; Wake, Hidenori; Hisaoka-Nakashima, Kazue; Nishibori, Masahiro; Nakata, Yoshihiro.

In: Journal of Neurochemistry, Vol. 150, No. 6, 01.09.2019, p. 738-758.

Research output: Contribution to journalArticle

Morioka, Norimitsu ; Miyauchi, Kazuki ; Miyashita, Keita ; Kochi, Takahiro ; Zhang, Fang Fang ; Nakamura, Yoki ; Liu, Keyue ; Wake, Hidenori ; Hisaoka-Nakashima, Kazue ; Nishibori, Masahiro ; Nakata, Yoshihiro. / Spinal high-mobility group box-1 induces long-lasting mechanical hypersensitivity through the toll-like receptor 4 and upregulation of interleukin-1β in activated astrocytes. In: Journal of Neurochemistry. 2019 ; Vol. 150, No. 6. pp. 738-758.
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abstract = "Intrathecal treatment with recombinant high-mobility group box-1 (rHMGB1) in na{\"i}ve mice leads to a persistent and significantly decreased hind paw withdrawal threshold to mechanical stimuli, suggesting that spinal HMGB1 evokes abnormal pain processing. By contrast, repeated intrathecal treatment with anti-HMGB1 antibody significantly reverses hind paw mechano-hypersensitivity in mice with a partial sciatic nerve ligation (PSNL). By contrast, the cellular mechanism by which spinal HMGB1 induces neuropathic pain has yet to be fully elaborated. The current study tested the hypothesis that spinal HMGB1 could induce mechanical hypersensitivity through the activation of specific receptor in glial cells. Intrathecal pretreatment with toll-like receptor (TLR) 4 inhibitors, but not TLR5, receptor for advanced glycation end-products and C-X-C chemokine receptor type 4 inhibitors, prevented rHMGB1-evoked mechanical hypersensitivity. Activation of spinal astrocytes appears to be crucial for the mechanism of action of rHMGB1 in na{\"i}ve mice, as intrathecal pretreatment with astrocytic inhibitors prevented the rHMGB1-induced mechanical hypersensitivity. Interleukin-1β (IL-1β) was up-regulated within activated astrocytes and block of TLR4 prevented the upregulation of IL-1β. Interleukin-1β appears to be secreted by activated astrocytes, as IL-1β neutralizing antibody prevented rHMGB1-induced mechanical hypersensitivity. Furthermore, intrathecal pretreatment with either MK801 or gabapentin prevented the rHMGB1-induced mechanical hypersensitivity, suggesting roles for spinal glutamate and the N-methyl-d-aspartate receptor in the mediation of rHMGB1-induced mechanical hypersensitivity. Thus, the current findings suggest that spinal HMGB1 upregulates IL-1β in spinal astrocytes through a TLR4-dependent pathway and increases glutamatergic nociceptive transduction. These spinal mechanisms could be key steps that maintain neuropathic pain. (Figure presented.).",
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AU - Kochi, Takahiro

AU - Zhang, Fang Fang

AU - Nakamura, Yoki

AU - Liu, Keyue

AU - Wake, Hidenori

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