Spin trapping of a free radical intermediate formed during microsomal metabolism of hydrazine

Atsuko Noda, Hiroshi Noda, Kohji Ohno, Toshiaki Sendo, Ayako Misaka, Yohko Kanazawa, Ryu ichi Isobe, Masaharu Hirata

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A radical formed during oxidative metabolism of hydrazine in rat liver microsomes was spin-trapped with α-phenyl-t-butylnitrone. The trapped species was identified as hydrazine radical by comparison of its ESR parameters and mass spectrum with those of the adduct formed during CuCl2 catalyzed oxidation of hydrazine. The requirement for oxygen and NADPH in the microsomal oxidation and the occurrence of a typical binding spectrum by difference spectroscopy suggest the involvement of the participation of the cytochrome P-450 enzyme system in the formation of hydrazine radical which must be a precursor of diimide during microsomal oxidation of hydrazine.

Original languageEnglish
Pages (from-to)1086-1091
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume133
Issue number3
DOIs
Publication statusPublished - Dec 31 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Spin trapping of a free radical intermediate formed during microsomal metabolism of hydrazine'. Together they form a unique fingerprint.

  • Cite this