Spin trapping of a free radical intermediate formed during microsomal metabolism of hydrazine

Atsuko Noda; Hiroshi Noda; Kohji Ohno; Toshiaki Sendo; Ayako Misaka; Yohko Kanazawa; Ryu ichi Isobe; Masaharu Hirata

doi:10.1016/0006-291X(85)91247-1

Spin trapping of a free radical intermediate formed during microsomal metabolism of hydrazine

Atsuko Noda, Hiroshi Noda, Kohji Ohno, Toshiaki Sendo, Ayako Misaka, Yohko Kanazawa, Ryu ichi Isobe, Masaharu Hirata

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

A radical formed during oxidative metabolism of hydrazine in rat liver microsomes was spin-trapped with α-phenyl-t-butylnitrone. The trapped species was identified as hydrazine radical by comparison of its ESR parameters and mass spectrum with those of the adduct formed during CuCl₂ catalyzed oxidation of hydrazine. The requirement for oxygen and NADPH in the microsomal oxidation and the occurrence of a typical binding spectrum by difference spectroscopy suggest the involvement of the participation of the cytochrome P-450 enzyme system in the formation of hydrazine radical which must be a precursor of diimide during microsomal oxidation of hydrazine.

Original language	English
Pages (from-to)	1086-1091
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	133
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(85)91247-1
Publication status	Published - Dec 31 1985
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Spin trapping of a free radical intermediate formed during microsomal metabolism of hydrazine. / Noda, Atsuko; Noda, Hiroshi; Ohno, Kohji; Sendo, Toshiaki; Misaka, Ayako; Kanazawa, Yohko; Isobe, Ryu ichi; Hirata, Masaharu.

In: Biochemical and Biophysical Research Communications, Vol. 133, No. 3, 31.12.1985, p. 1086-1091.

Research output: Contribution to journal › Article › peer-review

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abstract = "A radical formed during oxidative metabolism of hydrazine in rat liver microsomes was spin-trapped with α-phenyl-t-butylnitrone. The trapped species was identified as hydrazine radical by comparison of its ESR parameters and mass spectrum with those of the adduct formed during CuCl2 catalyzed oxidation of hydrazine. The requirement for oxygen and NADPH in the microsomal oxidation and the occurrence of a typical binding spectrum by difference spectroscopy suggest the involvement of the participation of the cytochrome P-450 enzyme system in the formation of hydrazine radical which must be a precursor of diimide during microsomal oxidation of hydrazine.",

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AU - Noda, Atsuko

AU - Noda, Hiroshi

AU - Ohno, Kohji

AU - Sendo, Toshiaki

AU - Misaka, Ayako

AU - Kanazawa, Yohko

AU - Isobe, Ryu ichi

AU - Hirata, Masaharu

PY - 1985/12/31

Y1 - 1985/12/31

N2 - A radical formed during oxidative metabolism of hydrazine in rat liver microsomes was spin-trapped with α-phenyl-t-butylnitrone. The trapped species was identified as hydrazine radical by comparison of its ESR parameters and mass spectrum with those of the adduct formed during CuCl2 catalyzed oxidation of hydrazine. The requirement for oxygen and NADPH in the microsomal oxidation and the occurrence of a typical binding spectrum by difference spectroscopy suggest the involvement of the participation of the cytochrome P-450 enzyme system in the formation of hydrazine radical which must be a precursor of diimide during microsomal oxidation of hydrazine.

AB - A radical formed during oxidative metabolism of hydrazine in rat liver microsomes was spin-trapped with α-phenyl-t-butylnitrone. The trapped species was identified as hydrazine radical by comparison of its ESR parameters and mass spectrum with those of the adduct formed during CuCl2 catalyzed oxidation of hydrazine. The requirement for oxygen and NADPH in the microsomal oxidation and the occurrence of a typical binding spectrum by difference spectroscopy suggest the involvement of the participation of the cytochrome P-450 enzyme system in the formation of hydrazine radical which must be a precursor of diimide during microsomal oxidation of hydrazine.

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Spin trapping of a free radical intermediate formed during microsomal metabolism of hydrazine

Abstract

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