Spin label study of the process of oxygen release by pea chloroplasts

A. V. Kulikov, Ye I. Yudanova, G. I. Likhtenshtein, S. I. Allakhverdiyev, V. V. Klimov

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Nitroxyl fatty acid probes inserted into the chloroplast membranes have been used to study the process of evolution of oxygen by chloroplasts. From the kinetics of the reduction of the nitroxyls on exposure to continuous illumination at two values of u.h.f. power the authors have determined the products ke[O2] where ke is the spin exchange constant of nitroxyl and oxygen; [O2] is the oxygen concentration. It is shown that unlike liposomes, in the chloroplast membranes there is no oxygen in the dark. This is possible as a result of oxygen uptake in the different biochemical processes. On illumination of the chloroplasts the oxygen evolved is detected, the maximum value ke[O2]=1·2 × 105 sec-1 being observed at the centre of the membrane. At temperatures above 40 and below -20 °C oxygen is not evolved.

    Original languageEnglish
    Pages (from-to)1055-1060
    Number of pages6
    JournalBiophysics
    Volume33
    Issue number6
    Publication statusPublished - Dec 1 1988

    ASJC Scopus subject areas

    • Biophysics

    Fingerprint

    Dive into the research topics of 'Spin label study of the process of oxygen release by pea chloroplasts'. Together they form a unique fingerprint.

    Cite this