Spermidine synthase genes are essential for survival of arabidopsis

Akihiro Imai, Takashi Matsuyama, Yoshie Hanzawa, Takashi Akiyama, Masanori Tamaoki, Hikaru Saji, Yumiko Shirano, Tomohiko Kato, Hiroaki Hayashi, Daisuke Shibata, Satoshi Tabata, Yoshibumi Komeda, Taku Takahashi

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

The cellular polyamines putrescine, spermidine, and spermine are ubiquitous in nature and have been implicated in a wide range of growth and developmental processes. There is little information, however, on mutant plants or animals defective in the synthesis of polyamines. The Arabidopsis genome has two genes encoding spermidine synthase, SPDS1 and SPDS2. In this paper, we describe T-DNA insertion mutants of both of these genes. While each mutant allele shows normal growth, spds1-1 spds2-1 double-mutant seeds are abnormally shrunken and they have embryos that are arrested morphologically at the heart-torpedo transition stage. These seeds contain significantly reduced levels of spermidine and high levels of its precursor, putrescine. The embryo lethal phenotype of spds1-1 spds2-1 is complemented by the wild-type SPDS1 gene. In addition, we observed a nearly identical seed phenotype among an F2 seed population from the cross between the spds2-1 allele and SPDS1 RNA interference transgenic lines. These data provide the first genetic evidence indicating a critical role of the spermidine synthase in plant embryo development.

Original languageEnglish
Pages (from-to)1565-1573
Number of pages9
JournalPlant Physiology
Volume135
Issue number3
DOIs
Publication statusPublished - Jul 2004

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Spermidine Synthase
Essential Genes
Arabidopsis
Seeds
mutants
spermidine
putrescine
polyamines
seeds
embryo (plant)
Putrescine
Spermidine
Polyamines
genes
alleles
phenotype
Embryonic Structures
Torpedo
Alleles
spermine

ASJC Scopus subject areas

  • Plant Science

Cite this

Imai, A., Matsuyama, T., Hanzawa, Y., Akiyama, T., Tamaoki, M., Saji, H., ... Takahashi, T. (2004). Spermidine synthase genes are essential for survival of arabidopsis. Plant Physiology, 135(3), 1565-1573. https://doi.org/10.1104/pp.104.041699

Spermidine synthase genes are essential for survival of arabidopsis. / Imai, Akihiro; Matsuyama, Takashi; Hanzawa, Yoshie; Akiyama, Takashi; Tamaoki, Masanori; Saji, Hikaru; Shirano, Yumiko; Kato, Tomohiko; Hayashi, Hiroaki; Shibata, Daisuke; Tabata, Satoshi; Komeda, Yoshibumi; Takahashi, Taku.

In: Plant Physiology, Vol. 135, No. 3, 07.2004, p. 1565-1573.

Research output: Contribution to journalArticle

Imai, A, Matsuyama, T, Hanzawa, Y, Akiyama, T, Tamaoki, M, Saji, H, Shirano, Y, Kato, T, Hayashi, H, Shibata, D, Tabata, S, Komeda, Y & Takahashi, T 2004, 'Spermidine synthase genes are essential for survival of arabidopsis', Plant Physiology, vol. 135, no. 3, pp. 1565-1573. https://doi.org/10.1104/pp.104.041699
Imai A, Matsuyama T, Hanzawa Y, Akiyama T, Tamaoki M, Saji H et al. Spermidine synthase genes are essential for survival of arabidopsis. Plant Physiology. 2004 Jul;135(3):1565-1573. https://doi.org/10.1104/pp.104.041699
Imai, Akihiro ; Matsuyama, Takashi ; Hanzawa, Yoshie ; Akiyama, Takashi ; Tamaoki, Masanori ; Saji, Hikaru ; Shirano, Yumiko ; Kato, Tomohiko ; Hayashi, Hiroaki ; Shibata, Daisuke ; Tabata, Satoshi ; Komeda, Yoshibumi ; Takahashi, Taku. / Spermidine synthase genes are essential for survival of arabidopsis. In: Plant Physiology. 2004 ; Vol. 135, No. 3. pp. 1565-1573.
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