Spermatogenesis arrest caused by conditional deletion of Hsp90α in adult mice

Chiaki Kajiwara, Shiho Kondo, Shizuha Uda, Lei Dai, Tomoko Ichiyanagi, Tomoki Chiba, Satoshi Ishido, Takehiko Koji, Heiichiro Udono

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

It is controversial whether a functional androgen receptor (AR) on germ cells, including spermatogonia, is essential for their development into sperm and, thus, initiation and maintenance of spermatogenesis. It was recently shown that many spermatocytes underwent apoptosis in the testes of Hsp90α KO mice. We had generated Hsp90α KO mice independently and confirmed this phenotype. However, the important question of whether Hsp90α is required to maintain spermatogenesis in adult mice in which testicular maturation is already completed could not be addressed using these conventional KO mice. To answer this question, we generated a tamoxifen-inducible deletion mutant of Hsp90α and found that conditional deletion of Hsp90α in adult mice caused even more severe apoptosis in germ cells beyond the pachytene stage, leading to complete arrest of spermatogenesis and testicular atrophy. Importantly, immunohistochemical analysis revealed that AR expression in WT testis was more evident in spermatogonia than in spermatocytes, whereas its expression was aberrant and ectopic in Hsp90α KO testis, raising the possibility that an AR abnormality in primordial germ cells is involved in spermatogenesis arrest in the Hsp90α KO mice. Our results suggest that the AR, specifically chaperoned by Hsp90α in spermatogonia, is critical for maintenance of established spermatogenesis and for survival of spermatocytes in adult testis, in addition to setting the first wave of spermatogenesis before puberty.

Original languageEnglish
Pages (from-to)977-982
Number of pages6
JournalBiology Open
Volume1
Issue number10
DOIs
Publication statusPublished - Oct 15 2012

Keywords

  • Androgen receptor
  • Hsp90α
  • Spermatogenesis
  • Spermatogonia

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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    Kajiwara, C., Kondo, S., Uda, S., Dai, L., Ichiyanagi, T., Chiba, T., Ishido, S., Koji, T., & Udono, H. (2012). Spermatogenesis arrest caused by conditional deletion of Hsp90α in adult mice. Biology Open, 1(10), 977-982. https://doi.org/10.1242/bio.2012646