Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II

Takahiro Ito, Qihong Xu, Hideaki Takeuchi, Takeo Kubo, Shunji Natori

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Previously, we characterized a rat cDNA for testis-specific transcription elongation factor S-II (SII-T1) (Q. Xu et al., J. Biol. Chem. 269, 3100-3103 (1994)). Here, we isolated a 335-bp fragment of the cDNA for mouse SII-T1, and used it to examine the expression of the SII-T1 gene in the testis by in situ hybridization. The results indicated that the SII-T1 gene is expressed exclusively in spermatocytes, showing no appreciable expression in spermatogonia, spermatids, or Leydig cells. RT-PCR experiments using testis RNA from W/W(v) mutant mice also suggested that SII-T1 is a specific transcription elongation factor essential for spermatogenesis.

Original languageEnglish
Pages (from-to)21-24
Number of pages4
JournalFEBS Letters
Volume385
Issue number1-2
DOIs
Publication statusPublished - Apr 29 1996
Externally publishedYes

Keywords

  • S-II
  • SII-T1
  • Spermatocytes
  • Spermatogenesis
  • Transcription elongation factor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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