Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II

Takahiro Ito, Qihong Xu, Hideaki Takeuchi, Takeo Kubo, Shunji Natori

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Previously, we characterized a rat cDNA for testis-specific transcription elongation factor S-II (SII-T1) (Q. Xu et al., J. Biol. Chem. 269, 3100-3103 (1994)). Here, we isolated a 335-bp fragment of the cDNA for mouse SII-T1, and used it to examine the expression of the SII-T1 gene in the testis by in situ hybridization. The results indicated that the SII-T1 gene is expressed exclusively in spermatocytes, showing no appreciable expression in spermatogonia, spermatids, or Leydig cells. RT-PCR experiments using testis RNA from W/W(v) mutant mice also suggested that SII-T1 is a specific transcription elongation factor essential for spermatogenesis.

Original languageEnglish
Pages (from-to)21-24
Number of pages4
JournalFEBS Letters
Volume385
Issue number1-2
DOIs
Publication statusPublished - Apr 29 1996
Externally publishedYes

Fingerprint

Spermatocytes
Testis
Complementary DNA
Genes
Peptide Elongation Factors
Gene Expression
Transcription
Rats
Spermatogonia
Spermatids
Leydig Cells
RNA
Spermatogenesis
In Situ Hybridization
Transcription Factors
Polymerase Chain Reaction
Experiments
transcription factor S-II

Keywords

  • S-II
  • SII-T1
  • Spermatocytes
  • Spermatogenesis
  • Transcription elongation factor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II. / Ito, Takahiro; Xu, Qihong; Takeuchi, Hideaki; Kubo, Takeo; Natori, Shunji.

In: FEBS Letters, Vol. 385, No. 1-2, 29.04.1996, p. 21-24.

Research output: Contribution to journalArticle

Ito, Takahiro ; Xu, Qihong ; Takeuchi, Hideaki ; Kubo, Takeo ; Natori, Shunji. / Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II. In: FEBS Letters. 1996 ; Vol. 385, No. 1-2. pp. 21-24.
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