Spectroscopic properties of LH2 from Thermochromatium tepidum in liposome and detergent micelles

Yilin Huo, Ying Shi, Qinyue Wang, Luoyuan Li, Long-Jiang Yu, Peng Wang, Jianping Zhang, Zhengyu Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

By the use of dynamic light scattering(DLS), transmission electron microscopy(TEM), UV-Vis and resonance Raman spectroscopy, the properties of LH2 from Thermochromatium(Tch.) tepidum in two detergent micelles and in liposome were studied, respectively. The results show that in LH2-liposome the conformation of spirilloxanthin, an incorporated longer chain carotenoid, is obviously different from that in detergent micelles. The charge state of the terminal groups of detergents or lipids is the key factor affecting the B850 absorption. Generally, the calcium ions cause red-shift of the B850 absorption and hyperchromicity, while the proton has the opposite effect. Based on the amino acid sequence analysis, several amino acid residues on the C-terminus of α apo-protein were proposed to constitute the potential binding site for the calcium ion and proton. Such Ca2+- and H+-regulated changes of B850 absorption might be an adaptive mechanism to the living environment for this species.

Original languageEnglish
Pages (from-to)1678-1685
Number of pages8
JournalGaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities
Volume37
Issue number9
DOIs
Publication statusPublished - Sep 10 2016

Fingerprint

Micelles
Liposomes
Detergents
Protons
Ions
Calcium
Amino Acids
Dynamic light scattering
Carotenoids
Raman spectroscopy
Conformations
Binding Sites
Transmission electron microscopy
Lipids
Proteins

Keywords

  • Detergent
  • Liposome
  • Peripheral antenna complex LH2
  • Thermochromatium tepidum

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Spectroscopic properties of LH2 from Thermochromatium tepidum in liposome and detergent micelles. / Huo, Yilin; Shi, Ying; Wang, Qinyue; Li, Luoyuan; Yu, Long-Jiang; Wang, Peng; Zhang, Jianping; Wang, Zhengyu.

In: Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities, Vol. 37, No. 9, 10.09.2016, p. 1678-1685.

Research output: Contribution to journalArticle

Huo, Yilin ; Shi, Ying ; Wang, Qinyue ; Li, Luoyuan ; Yu, Long-Jiang ; Wang, Peng ; Zhang, Jianping ; Wang, Zhengyu. / Spectroscopic properties of LH2 from Thermochromatium tepidum in liposome and detergent micelles. In: Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities. 2016 ; Vol. 37, No. 9. pp. 1678-1685.
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AU - Zhang, Jianping

AU - Wang, Zhengyu

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AB - By the use of dynamic light scattering(DLS), transmission electron microscopy(TEM), UV-Vis and resonance Raman spectroscopy, the properties of LH2 from Thermochromatium(Tch.) tepidum in two detergent micelles and in liposome were studied, respectively. The results show that in LH2-liposome the conformation of spirilloxanthin, an incorporated longer chain carotenoid, is obviously different from that in detergent micelles. The charge state of the terminal groups of detergents or lipids is the key factor affecting the B850 absorption. Generally, the calcium ions cause red-shift of the B850 absorption and hyperchromicity, while the proton has the opposite effect. Based on the amino acid sequence analysis, several amino acid residues on the C-terminus of α apo-protein were proposed to constitute the potential binding site for the calcium ion and proton. Such Ca2+- and H+-regulated changes of B850 absorption might be an adaptive mechanism to the living environment for this species.

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