Spectroscopic Analysis and Electron Microscopic Observation of Lutein Dispersed in Bile Acid Solutions

Shigeaki Takagi, Takayuki Ito, Akio Nakajima, Yoshinobu Kimura, Kunio Takeda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Spectroscopic and electron microscopic studies of lutein dispersed in aqueous solutions of the bile acids, sodium cholate (CA), sodium deoxycholate (DCA), and sodium chenodeoxycholate (CDCA) were done. The bile acids caused the lutein molecules to form water-soluble aggregates of right-handed helices, which gave characteristic ellipticities in the wavelength range of 350-450 nm. The molar ellipticity of lutein increased during aggregation. The ellipticities of lutein wax maximum at about 26 mM CA, 3 mM DCA, and 7 mM CDCA. The maximum ellipticity of lutein in CA was larger than those in the two other bile acids. This means that CA makes more regular lutein aggregate than DCA or CDCA. The binding ratio of CA to lutein was estimated to be 1000-3000 mol/mol, but that of DCA to lutein was 80 mol/mol. In the CA solution, large rod-shaped lutein aggregates 1.5-3μm long and 0.2-0.8μm in diameter were observed under the electron microscope. Small spherical particles 0.3μm in diameter adhered to the lutein aggregates. The number of these particles increased as the CA concentration increased, indicating that they were CA micelles. Therefore, lutein seemed to interact with the CA micelles.

Original languageEnglish
Pages (from-to)1055-1060
Number of pages6
JournalNippon Nogeikagaku Kaishi
Volume67
Issue number7
DOIs
Publication statusPublished - 1993

Fingerprint

Lutein
Spectroscopic analysis
bile acids
Cholates
Bile Acids and Salts
lutein
electrons
Electrons
deoxycholic acid
Deoxycholic Acid
Chenodeoxycholic Acid
Micelles
micelles
sodium
Sodium Cholate
Waxes
electron microscopes
waxes
aqueous solutions
wavelengths

ASJC Scopus subject areas

  • Biotechnology
  • Chemistry (miscellaneous)
  • Medicine (miscellaneous)
  • Food Science

Cite this

Spectroscopic Analysis and Electron Microscopic Observation of Lutein Dispersed in Bile Acid Solutions. / Takagi, Shigeaki; Ito, Takayuki; Nakajima, Akio; Kimura, Yoshinobu; Takeda, Kunio.

In: Nippon Nogeikagaku Kaishi, Vol. 67, No. 7, 1993, p. 1055-1060.

Research output: Contribution to journalArticle

Takagi, Shigeaki ; Ito, Takayuki ; Nakajima, Akio ; Kimura, Yoshinobu ; Takeda, Kunio. / Spectroscopic Analysis and Electron Microscopic Observation of Lutein Dispersed in Bile Acid Solutions. In: Nippon Nogeikagaku Kaishi. 1993 ; Vol. 67, No. 7. pp. 1055-1060.
@article{7c4973fa770344a3891d958cefa06871,
title = "Spectroscopic Analysis and Electron Microscopic Observation of Lutein Dispersed in Bile Acid Solutions",
abstract = "Spectroscopic and electron microscopic studies of lutein dispersed in aqueous solutions of the bile acids, sodium cholate (CA), sodium deoxycholate (DCA), and sodium chenodeoxycholate (CDCA) were done. The bile acids caused the lutein molecules to form water-soluble aggregates of right-handed helices, which gave characteristic ellipticities in the wavelength range of 350-450 nm. The molar ellipticity of lutein increased during aggregation. The ellipticities of lutein wax maximum at about 26 mM CA, 3 mM DCA, and 7 mM CDCA. The maximum ellipticity of lutein in CA was larger than those in the two other bile acids. This means that CA makes more regular lutein aggregate than DCA or CDCA. The binding ratio of CA to lutein was estimated to be 1000-3000 mol/mol, but that of DCA to lutein was 80 mol/mol. In the CA solution, large rod-shaped lutein aggregates 1.5-3μm long and 0.2-0.8μm in diameter were observed under the electron microscope. Small spherical particles 0.3μm in diameter adhered to the lutein aggregates. The number of these particles increased as the CA concentration increased, indicating that they were CA micelles. Therefore, lutein seemed to interact with the CA micelles.",
author = "Shigeaki Takagi and Takayuki Ito and Akio Nakajima and Yoshinobu Kimura and Kunio Takeda",
year = "1993",
doi = "10.1271/nogeikagaku1924.67.1055",
language = "English",
volume = "67",
pages = "1055--1060",
journal = "Nippon Nogeikagaku Kaishi",
issn = "0002-1407",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "7",

}

TY - JOUR

T1 - Spectroscopic Analysis and Electron Microscopic Observation of Lutein Dispersed in Bile Acid Solutions

AU - Takagi, Shigeaki

AU - Ito, Takayuki

AU - Nakajima, Akio

AU - Kimura, Yoshinobu

AU - Takeda, Kunio

PY - 1993

Y1 - 1993

N2 - Spectroscopic and electron microscopic studies of lutein dispersed in aqueous solutions of the bile acids, sodium cholate (CA), sodium deoxycholate (DCA), and sodium chenodeoxycholate (CDCA) were done. The bile acids caused the lutein molecules to form water-soluble aggregates of right-handed helices, which gave characteristic ellipticities in the wavelength range of 350-450 nm. The molar ellipticity of lutein increased during aggregation. The ellipticities of lutein wax maximum at about 26 mM CA, 3 mM DCA, and 7 mM CDCA. The maximum ellipticity of lutein in CA was larger than those in the two other bile acids. This means that CA makes more regular lutein aggregate than DCA or CDCA. The binding ratio of CA to lutein was estimated to be 1000-3000 mol/mol, but that of DCA to lutein was 80 mol/mol. In the CA solution, large rod-shaped lutein aggregates 1.5-3μm long and 0.2-0.8μm in diameter were observed under the electron microscope. Small spherical particles 0.3μm in diameter adhered to the lutein aggregates. The number of these particles increased as the CA concentration increased, indicating that they were CA micelles. Therefore, lutein seemed to interact with the CA micelles.

AB - Spectroscopic and electron microscopic studies of lutein dispersed in aqueous solutions of the bile acids, sodium cholate (CA), sodium deoxycholate (DCA), and sodium chenodeoxycholate (CDCA) were done. The bile acids caused the lutein molecules to form water-soluble aggregates of right-handed helices, which gave characteristic ellipticities in the wavelength range of 350-450 nm. The molar ellipticity of lutein increased during aggregation. The ellipticities of lutein wax maximum at about 26 mM CA, 3 mM DCA, and 7 mM CDCA. The maximum ellipticity of lutein in CA was larger than those in the two other bile acids. This means that CA makes more regular lutein aggregate than DCA or CDCA. The binding ratio of CA to lutein was estimated to be 1000-3000 mol/mol, but that of DCA to lutein was 80 mol/mol. In the CA solution, large rod-shaped lutein aggregates 1.5-3μm long and 0.2-0.8μm in diameter were observed under the electron microscope. Small spherical particles 0.3μm in diameter adhered to the lutein aggregates. The number of these particles increased as the CA concentration increased, indicating that they were CA micelles. Therefore, lutein seemed to interact with the CA micelles.

UR - http://www.scopus.com/inward/record.url?scp=33644568397&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644568397&partnerID=8YFLogxK

U2 - 10.1271/nogeikagaku1924.67.1055

DO - 10.1271/nogeikagaku1924.67.1055

M3 - Article

AN - SCOPUS:33644568397

VL - 67

SP - 1055

EP - 1060

JO - Nippon Nogeikagaku Kaishi

JF - Nippon Nogeikagaku Kaishi

SN - 0002-1407

IS - 7

ER -