Spectrophotometric system based on a device created by 3D printing for the accommodation of a webcam chamber as a detection system

Kaewta Danchana, Piyawan Phansi, Cheilane T. de Souza, Sergio L.C. Ferreira, Víctor Cerdà

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The development of a simple and economical spectrophotometric system based on the use of a device created by 3D printing and the electronics necessary to control the intensity of the radiation source was described. The measurements are made with a low-cost digital webcam. The entire system is only powered through the USB outputs of a computer, which makes the portable and really practical system for the measurements in the field. This method was applied to determine iron (II) in waters using o-phenanthroline as chromogenic reagent giving a red complex, and also to hypochlorite determination using tetramethylbenzidine as the reagent providing a yellow color. The calibration curves were built using a mathematical algorithm making a RGB deconvolution. The intense of colors obtained from a webcam in each concentration of analyte had a relationship with the absorbance values. In order to confirm the accuracy and precision of this method, a traditional spectrophotometer was used for validation.

Original languageEnglish
Article number120250
JournalTalanta
Volume206
DOIs
Publication statusPublished - Jan 1 2020
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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