Spectrophotometric determination of urinary protein with o-sulfophenylfluorone-metal complex

Takako Yamaguchi; Emi Amano; Shinichiro Kamino; Satoko Umehara; Chizuko Yanaihara; Yoshikazu Fujita

doi:10.2116/analsci.21.1237

Spectrophotometric determination of urinary protein with o-sulfophenylfluorone-metal complex

Takako Yamaguchi, Emi Amano, Shinichiro Kamino, Satoko Umehara, Chizuko Yanaihara, Yoshikazu Fujita

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A simple and sensitive spectrophotometric method for the determination of human serum albumin (HSA) was established based on the ternary complex-formation reaction of HSA with o-sulfophenylfluorone (SPF) as a xanthene dye and metal ion (niobium(V) and bismuth(III)) in the presence of a dispersion agent. This new method enabled the determination of HSA in the range of 1-15 μg/ml HSA by measuring the difference of the absorbance at 530 nm between HSA-SPF-metal ion and SPF-metal ion solutions. In the determination of HSA, this method is about 2-times more sensitive than the Pyrogallol Red-molybdenum(VI) method (PR method), which accounts for more than 80% of the quantification methods for urinary protein assays in Japan. There was no significant difference between the results obtained by the present method and the PR method for human urine samples. The binding process between the SPF-metal complex and HSA was studied by determining the binding parameters and the thermodynamic parameters.

Original language	English
Pages (from-to)	1237-1240
Number of pages	4
Journal	Analytical Sciences
Volume	21
Issue number	10
DOIs	https://doi.org/10.2116/analsci.21.1237
Publication status	Published - Oct 2005
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

Access to Document

10.2116/analsci.21.1237

Cite this

@article{288e1343dee34a1b8fdd2d2787c3e34a,

title = "Spectrophotometric determination of urinary protein with o-sulfophenylfluorone-metal complex",

abstract = "A simple and sensitive spectrophotometric method for the determination of human serum albumin (HSA) was established based on the ternary complex-formation reaction of HSA with o-sulfophenylfluorone (SPF) as a xanthene dye and metal ion (niobium(V) and bismuth(III)) in the presence of a dispersion agent. This new method enabled the determination of HSA in the range of 1-15 μg/ml HSA by measuring the difference of the absorbance at 530 nm between HSA-SPF-metal ion and SPF-metal ion solutions. In the determination of HSA, this method is about 2-times more sensitive than the Pyrogallol Red-molybdenum(VI) method (PR method), which accounts for more than 80% of the quantification methods for urinary protein assays in Japan. There was no significant difference between the results obtained by the present method and the PR method for human urine samples. The binding process between the SPF-metal complex and HSA was studied by determining the binding parameters and the thermodynamic parameters.",

author = "Takako Yamaguchi and Emi Amano and Shinichiro Kamino and Satoko Umehara and Chizuko Yanaihara and Yoshikazu Fujita",

year = "2005",

month = oct,

doi = "10.2116/analsci.21.1237",

language = "English",

volume = "21",

pages = "1237--1240",

journal = "Analytical Sciences",

issn = "0910-6340",

publisher = "Japan Society for Analytical Chemistry",

number = "10",

}

TY - JOUR

T1 - Spectrophotometric determination of urinary protein with o-sulfophenylfluorone-metal complex

AU - Yamaguchi, Takako

AU - Amano, Emi

AU - Kamino, Shinichiro

AU - Umehara, Satoko

AU - Yanaihara, Chizuko

AU - Fujita, Yoshikazu

PY - 2005/10

Y1 - 2005/10

N2 - A simple and sensitive spectrophotometric method for the determination of human serum albumin (HSA) was established based on the ternary complex-formation reaction of HSA with o-sulfophenylfluorone (SPF) as a xanthene dye and metal ion (niobium(V) and bismuth(III)) in the presence of a dispersion agent. This new method enabled the determination of HSA in the range of 1-15 μg/ml HSA by measuring the difference of the absorbance at 530 nm between HSA-SPF-metal ion and SPF-metal ion solutions. In the determination of HSA, this method is about 2-times more sensitive than the Pyrogallol Red-molybdenum(VI) method (PR method), which accounts for more than 80% of the quantification methods for urinary protein assays in Japan. There was no significant difference between the results obtained by the present method and the PR method for human urine samples. The binding process between the SPF-metal complex and HSA was studied by determining the binding parameters and the thermodynamic parameters.

AB - A simple and sensitive spectrophotometric method for the determination of human serum albumin (HSA) was established based on the ternary complex-formation reaction of HSA with o-sulfophenylfluorone (SPF) as a xanthene dye and metal ion (niobium(V) and bismuth(III)) in the presence of a dispersion agent. This new method enabled the determination of HSA in the range of 1-15 μg/ml HSA by measuring the difference of the absorbance at 530 nm between HSA-SPF-metal ion and SPF-metal ion solutions. In the determination of HSA, this method is about 2-times more sensitive than the Pyrogallol Red-molybdenum(VI) method (PR method), which accounts for more than 80% of the quantification methods for urinary protein assays in Japan. There was no significant difference between the results obtained by the present method and the PR method for human urine samples. The binding process between the SPF-metal complex and HSA was studied by determining the binding parameters and the thermodynamic parameters.

UR - http://www.scopus.com/inward/record.url?scp=27644445143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27644445143&partnerID=8YFLogxK

U2 - 10.2116/analsci.21.1237

DO - 10.2116/analsci.21.1237

M3 - Article

C2 - 16270586

AN - SCOPUS:27644445143

SN - 0910-6340

VL - 21

SP - 1237

EP - 1240

JO - Analytical Sciences

JF - Analytical Sciences

IS - 10

ER -

Spectrophotometric determination of urinary protein with o-sulfophenylfluorone-metal complex

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this