Spectrophotometric determination of uric acid based on fading of o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex

Takako Yamaguchi, Kohei Hasegawa, Shinichiro Kamino, Kanako Miyachi, Hiroshi Tominaga, Yoshikazu Fujita

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Abstract

A simple and highly sensitive spectrophotometric method for the determination of uric acid (UA) was established based on fading of the o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex. In the determination of UA, Beer's law is obeyed in the range of 0.01 - 0.20 μg ml-1, with an effective molar absorptivity at 635 nm, the relative standard deviation being 6.5 × 105 dm3 mol-1 cm-1 and 1.5% (n = 5). This method is about 20-times more sensitive than the conventional methods. The method was successfully applied to the assay of UA in human urine. 2007

Original languageEnglish
Pages (from-to)223-226
Number of pages4
JournalAnalytical Sciences
Volume23
Issue number2
DOIs
Publication statusPublished - Feb 2007
Externally publishedYes

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ASJC Scopus subject areas

  • Analytical Chemistry

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