Spectrophotometric determination of spermine and related compounds using o-hydroxyhydroquinonephthalein and manganese(II)

Kanako Miyachi, Kenzo Moriyama, Takako Yamaguchi, Hiroshi Tominaga, Shinichiro Kamino, Yoshikazu Fujita

Research output: Contribution to journalArticle

3 Citations (Scopus)


A simple and highly sensitive spectrophotometric method for the determination of spermine (Spm) was established based on the ternary complex formation reaction of Spm with o-hydroxyhydroquinonephthalein (QP) as a xanthene dye and manganese(II) as a metal ion in the presence of a dispersion agent. The apparent molar absorptivity at 555 nm and the relative standard deviation of the proposed method were 1.4 × 105 dm3 mol -1 cm-1 and 0.50% (n = 10), respectively. In the method for flow-injection analysis (FIA), which employs a single-channel flow manifold system, a good linear relationship was observed over the 2 - 20 pg μl -1 range of Spm by direct injection. 2007

Original languageEnglish
Pages (from-to)1103-1107
Number of pages5
JournalAnalytical Sciences
Issue number9
Publication statusPublished - Sep 2007
Externally publishedYes


ASJC Scopus subject areas

  • Analytical Chemistry

Cite this