A new iadirect spectrophotometric method for the determination of phosphate ion by the use of solid ferric salt of tetraphenylporphine-trisulfonic acid (TPPSFe) was developed. The method is based on the measurement of the absorbance of TPPS liberated from TPPSFe at its Q band (procedure A) or its Soret band (procedure B). Phosphate ion ranging from 10 to 50 pg and from 4 to 20μg could be determined based on the absorption coefficients of 8.2 x 103 at 512 nm (procedure A) and 2.2 x 105 at 434 nm (procedure B). The advantages of this method are its simple procedure and mild conditions. Procedure A was successfully applied to the determination of phosphate ion liberated from glucose-1-phosphate by acid phosphatase.
- acid phosphatase
- determination of phosphate ion
- ferric salt glucose-1 -phosphate
- indirect spectrophotometry
- tetraphenylporphine-trisulfonic acid
ASJC Scopus subject areas
- Drug Discovery