Spectrophotometric Determination of Phosphate Ion with Ferric Salt of Tetraphenylporphine-trisulfonic Acid

Yutaka Saito, Masaki Mifune, Kenji Ikegami, Kimiya Okazaki, Junichi Odo, Yoshimasa Tanaka

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A new iadirect spectrophotometric method for the determination of phosphate ion by the use of solid ferric salt of tetraphenylporphine-trisulfonic acid (TPPSFe) was developed. The method is based on the measurement of the absorbance of TPPS liberated from TPPSFe at its Q band (procedure A) or its Soret band (procedure B). Phosphate ion ranging from 10 to 50 pg and from 4 to 20μg could be determined based on the absorption coefficients of 8.2 x 103 at 512 nm (procedure A) and 2.2 x 105 at 434 nm (procedure B). The advantages of this method are its simple procedure and mild conditions. Procedure A was successfully applied to the determination of phosphate ion liberated from glucose-1-phosphate by acid phosphatase.

Original languageEnglish
Pages (from-to)3381-3384
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume33
Issue number8
DOIs
Publication statusPublished - 1985

Keywords

  • acid phosphatase
  • determination of phosphate ion
  • ferric salt glucose-1 -phosphate
  • indirect spectrophotometry
  • tetraphenylporphine-trisulfonic acid

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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