Spectrophotometric determination of manganese(II) and basic polyamino acids with o-sulfophenylfluorone

Shinya Kaji, Mayu Tomida, Mitsur u. Hoshino, Shinichiro Kamino, Mamiko Asano, Takako Yamaguchi, Yoshikazu Fujita

Research output: Contribution to journalArticle

Abstract

A spectrophotometric method was established for the determination of manganese(II) {Mn(II)} and Polylysine (PolyL) with o-sulfophenylfluorone (SPF). Beer's law was obeyed in the range of 0.06~3.3 μg for Mn(II) in a basic medium with an apparent molar absorptivity(ε) at 565 nm, the relative standard deviation (RSD) being 1.63×105 L mol-1 cm-1 and 1.46 ( (n =5). This method is about 2-70 times more sensitive than the existing spectrophptpmetric methods, and can determine more easily. Also, the method was successfully applied to assays of Mn(II) in real samples. The thermodynamic parameters suggest that the colored complex formed in this reaction system is an association complex between [Mn(II)=SPF] and [TSAC]. Next, Beer's law was obeyed in the range of 1.0~60 μg for PolyL, a basic solution. The ε and RSD are 4.18×106 L mol-1 cm-1 (molar weight≒225000) at 565 nm and 1.49 ( (n =5), respectively. This method can determine polymers, not monomers. Furthermore, this method was applied to recovery tests of PolyL in real samples; the results were satisfactory.

Original languageEnglish
Pages (from-to)619-627
Number of pages9
JournalBunseki Kagaku
Volume60
Issue number8
DOIs
Publication statusPublished - 2011

Fingerprint

Manganese
Polylysine
Acids
Assays
Polymers
Monomers
Association reactions
Thermodynamics
Recovery
o-sulfophenylfluorone

Keywords

  • Basic polyamino acid
  • Manganese(II) thermodynamic parameter
  • O-sulfophenylfluorone
  • Polylysine
  • Spectrophotometry

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Kaji, S., Tomida, M., Hoshino, M. U., Kamino, S., Asano, M., Yamaguchi, T., & Fujita, Y. (2011). Spectrophotometric determination of manganese(II) and basic polyamino acids with o-sulfophenylfluorone. Bunseki Kagaku, 60(8), 619-627. https://doi.org/10.2116/bunsekikagaku.60.619

Spectrophotometric determination of manganese(II) and basic polyamino acids with o-sulfophenylfluorone. / Kaji, Shinya; Tomida, Mayu; Hoshino, Mitsur u.; Kamino, Shinichiro; Asano, Mamiko; Yamaguchi, Takako; Fujita, Yoshikazu.

In: Bunseki Kagaku, Vol. 60, No. 8, 2011, p. 619-627.

Research output: Contribution to journalArticle

Kaji, S, Tomida, M, Hoshino, MU, Kamino, S, Asano, M, Yamaguchi, T & Fujita, Y 2011, 'Spectrophotometric determination of manganese(II) and basic polyamino acids with o-sulfophenylfluorone', Bunseki Kagaku, vol. 60, no. 8, pp. 619-627. https://doi.org/10.2116/bunsekikagaku.60.619
Kaji, Shinya ; Tomida, Mayu ; Hoshino, Mitsur u. ; Kamino, Shinichiro ; Asano, Mamiko ; Yamaguchi, Takako ; Fujita, Yoshikazu. / Spectrophotometric determination of manganese(II) and basic polyamino acids with o-sulfophenylfluorone. In: Bunseki Kagaku. 2011 ; Vol. 60, No. 8. pp. 619-627.
@article{1622994b2ba34c58a14ae88508e64e69,
title = "Spectrophotometric determination of manganese(II) and basic polyamino acids with o-sulfophenylfluorone",
abstract = "A spectrophotometric method was established for the determination of manganese(II) {Mn(II)} and Polylysine (PolyL) with o-sulfophenylfluorone (SPF). Beer's law was obeyed in the range of 0.06~3.3 μg for Mn(II) in a basic medium with an apparent molar absorptivity(ε) at 565 nm, the relative standard deviation (RSD) being 1.63×105 L mol-1 cm-1 and 1.46 ( (n =5). This method is about 2-70 times more sensitive than the existing spectrophptpmetric methods, and can determine more easily. Also, the method was successfully applied to assays of Mn(II) in real samples. The thermodynamic parameters suggest that the colored complex formed in this reaction system is an association complex between [Mn(II)=SPF] and [TSAC]. Next, Beer's law was obeyed in the range of 1.0~60 μg for PolyL, a basic solution. The ε and RSD are 4.18×106 L mol-1 cm-1 (molar weight≒225000) at 565 nm and 1.49 ( (n =5), respectively. This method can determine polymers, not monomers. Furthermore, this method was applied to recovery tests of PolyL in real samples; the results were satisfactory.",
keywords = "Basic polyamino acid, Manganese(II) thermodynamic parameter, O-sulfophenylfluorone, Polylysine, Spectrophotometry",
author = "Shinya Kaji and Mayu Tomida and Hoshino, {Mitsur u.} and Shinichiro Kamino and Mamiko Asano and Takako Yamaguchi and Yoshikazu Fujita",
year = "2011",
doi = "10.2116/bunsekikagaku.60.619",
language = "English",
volume = "60",
pages = "619--627",
journal = "Bunseki Kagaku",
issn = "0525-1931",
publisher = "Japan Society for Analytical Chemistry",
number = "8",

}

TY - JOUR

T1 - Spectrophotometric determination of manganese(II) and basic polyamino acids with o-sulfophenylfluorone

AU - Kaji, Shinya

AU - Tomida, Mayu

AU - Hoshino, Mitsur u.

AU - Kamino, Shinichiro

AU - Asano, Mamiko

AU - Yamaguchi, Takako

AU - Fujita, Yoshikazu

PY - 2011

Y1 - 2011

N2 - A spectrophotometric method was established for the determination of manganese(II) {Mn(II)} and Polylysine (PolyL) with o-sulfophenylfluorone (SPF). Beer's law was obeyed in the range of 0.06~3.3 μg for Mn(II) in a basic medium with an apparent molar absorptivity(ε) at 565 nm, the relative standard deviation (RSD) being 1.63×105 L mol-1 cm-1 and 1.46 ( (n =5). This method is about 2-70 times more sensitive than the existing spectrophptpmetric methods, and can determine more easily. Also, the method was successfully applied to assays of Mn(II) in real samples. The thermodynamic parameters suggest that the colored complex formed in this reaction system is an association complex between [Mn(II)=SPF] and [TSAC]. Next, Beer's law was obeyed in the range of 1.0~60 μg for PolyL, a basic solution. The ε and RSD are 4.18×106 L mol-1 cm-1 (molar weight≒225000) at 565 nm and 1.49 ( (n =5), respectively. This method can determine polymers, not monomers. Furthermore, this method was applied to recovery tests of PolyL in real samples; the results were satisfactory.

AB - A spectrophotometric method was established for the determination of manganese(II) {Mn(II)} and Polylysine (PolyL) with o-sulfophenylfluorone (SPF). Beer's law was obeyed in the range of 0.06~3.3 μg for Mn(II) in a basic medium with an apparent molar absorptivity(ε) at 565 nm, the relative standard deviation (RSD) being 1.63×105 L mol-1 cm-1 and 1.46 ( (n =5). This method is about 2-70 times more sensitive than the existing spectrophptpmetric methods, and can determine more easily. Also, the method was successfully applied to assays of Mn(II) in real samples. The thermodynamic parameters suggest that the colored complex formed in this reaction system is an association complex between [Mn(II)=SPF] and [TSAC]. Next, Beer's law was obeyed in the range of 1.0~60 μg for PolyL, a basic solution. The ε and RSD are 4.18×106 L mol-1 cm-1 (molar weight≒225000) at 565 nm and 1.49 ( (n =5), respectively. This method can determine polymers, not monomers. Furthermore, this method was applied to recovery tests of PolyL in real samples; the results were satisfactory.

KW - Basic polyamino acid

KW - Manganese(II) thermodynamic parameter

KW - O-sulfophenylfluorone

KW - Polylysine

KW - Spectrophotometry

UR - http://www.scopus.com/inward/record.url?scp=80053258716&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053258716&partnerID=8YFLogxK

U2 - 10.2116/bunsekikagaku.60.619

DO - 10.2116/bunsekikagaku.60.619

M3 - Article

AN - SCOPUS:80053258716

VL - 60

SP - 619

EP - 627

JO - Bunseki Kagaku

JF - Bunseki Kagaku

SN - 0525-1931

IS - 8

ER -