Spectrophotometric determination of insuline by ternary complex formation with o-carboxyphenylfluorone-copper(II) complex

Masahito Naito, Hiroto Nakashima, Masanobu Ohki, Shinichiro Kamino, Takako Yamaguchi, Yoshikazu Fujita

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The method is based on ternary complex formation among o- carboxyphenylfluorone, copper(II), and insuline. In the determination of insuline, Beer's law is obeyed in the range of 0.5∼50 μg mL -1. The effective molar absorptivity at 550 nm and the relative standard deviation were 8.2 × 10 4 dm 3 mol -1 cm -1 and 2.2% (n = 6), respectively. The proposed method should be useful for a simple determination of insuline.

Original languageEnglish
Pages (from-to)781-784
Number of pages4
JournalBunseki Kagaku
Volume56
Issue number9
DOIs
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Copper
4-carboxyphenylfluorone

Keywords

  • Insuline
  • Metabolic syndrome
  • o-carboxyphenylfluorone
  • Spectrophotometry
  • Ternary complex formation

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Spectrophotometric determination of insuline by ternary complex formation with o-carboxyphenylfluorone-copper(II) complex. / Naito, Masahito; Nakashima, Hiroto; Ohki, Masanobu; Kamino, Shinichiro; Yamaguchi, Takako; Fujita, Yoshikazu.

In: Bunseki Kagaku, Vol. 56, No. 9, 2007, p. 781-784.

Research output: Contribution to journalArticle

Naito, Masahito ; Nakashima, Hiroto ; Ohki, Masanobu ; Kamino, Shinichiro ; Yamaguchi, Takako ; Fujita, Yoshikazu. / Spectrophotometric determination of insuline by ternary complex formation with o-carboxyphenylfluorone-copper(II) complex. In: Bunseki Kagaku. 2007 ; Vol. 56, No. 9. pp. 781-784.
@article{53484623deae4860a1cfd048cf01dd50,
title = "Spectrophotometric determination of insuline by ternary complex formation with o-carboxyphenylfluorone-copper(II) complex",
abstract = "The method is based on ternary complex formation among o- carboxyphenylfluorone, copper(II), and insuline. In the determination of insuline, Beer's law is obeyed in the range of 0.5∼50 μg mL -1. The effective molar absorptivity at 550 nm and the relative standard deviation were 8.2 × 10 4 dm 3 mol -1 cm -1 and 2.2{\%} (n = 6), respectively. The proposed method should be useful for a simple determination of insuline.",
keywords = "Insuline, Metabolic syndrome, o-carboxyphenylfluorone, Spectrophotometry, Ternary complex formation",
author = "Masahito Naito and Hiroto Nakashima and Masanobu Ohki and Shinichiro Kamino and Takako Yamaguchi and Yoshikazu Fujita",
year = "2007",
doi = "10.2116/bunsekikagaku.56.781",
language = "English",
volume = "56",
pages = "781--784",
journal = "Bunseki Kagaku",
issn = "0525-1931",
publisher = "Japan Society for Analytical Chemistry",
number = "9",

}

TY - JOUR

T1 - Spectrophotometric determination of insuline by ternary complex formation with o-carboxyphenylfluorone-copper(II) complex

AU - Naito, Masahito

AU - Nakashima, Hiroto

AU - Ohki, Masanobu

AU - Kamino, Shinichiro

AU - Yamaguchi, Takako

AU - Fujita, Yoshikazu

PY - 2007

Y1 - 2007

N2 - The method is based on ternary complex formation among o- carboxyphenylfluorone, copper(II), and insuline. In the determination of insuline, Beer's law is obeyed in the range of 0.5∼50 μg mL -1. The effective molar absorptivity at 550 nm and the relative standard deviation were 8.2 × 10 4 dm 3 mol -1 cm -1 and 2.2% (n = 6), respectively. The proposed method should be useful for a simple determination of insuline.

AB - The method is based on ternary complex formation among o- carboxyphenylfluorone, copper(II), and insuline. In the determination of insuline, Beer's law is obeyed in the range of 0.5∼50 μg mL -1. The effective molar absorptivity at 550 nm and the relative standard deviation were 8.2 × 10 4 dm 3 mol -1 cm -1 and 2.2% (n = 6), respectively. The proposed method should be useful for a simple determination of insuline.

KW - Insuline

KW - Metabolic syndrome

KW - o-carboxyphenylfluorone

KW - Spectrophotometry

KW - Ternary complex formation

UR - http://www.scopus.com/inward/record.url?scp=36148965530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36148965530&partnerID=8YFLogxK

U2 - 10.2116/bunsekikagaku.56.781

DO - 10.2116/bunsekikagaku.56.781

M3 - Article

AN - SCOPUS:36148965530

VL - 56

SP - 781

EP - 784

JO - Bunseki Kagaku

JF - Bunseki Kagaku

SN - 0525-1931

IS - 9

ER -