Spectrophotometric determination of insuline by ternary complex formation with o-carboxyphenylfluorone-copper(II) complex

Masahito Naito, Hiroto Nakashima, Masanobu Ohki, Shinichirou Kamino, Takako Yamaguchi, Yoshikazu Fujita

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Abstract

The method is based on ternary complex formation among o- carboxyphenylfluorone, copper(II), and insuline. In the determination of insuline, Beer's law is obeyed in the range of 0.5∼50 μg mL -1. The effective molar absorptivity at 550 nm and the relative standard deviation were 8.2 × 10 4 dm 3 mol -1 cm -1 and 2.2% (n = 6), respectively. The proposed method should be useful for a simple determination of insuline.

Original languageEnglish
Pages (from-to)781-784
Number of pages4
JournalBUNSEKI KAGAKU
Volume56
Issue number9
DOIs
Publication statusPublished - Nov 21 2007

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Keywords

  • Insuline
  • Metabolic syndrome
  • Spectrophotometry
  • Ternary complex formation
  • o-carboxyphenylfluorone

ASJC Scopus subject areas

  • Analytical Chemistry

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