Spectral and functional studies on siphonaxanthin-type light-harvesting complex of photosystem II from Bryopsis corticulans

Wenda Wang, Xiaochun Qin, Min Sang, Dongqin Chen, Kebin Wang, Rongchen Lin, Congming Lu, Jian-Ren Shen, Tingyun Kuang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Carotenoids with conjugated carbonyl groups possess special photophysical properties which have been studied in some water-soluble light-harvesting proteins (Polívka and Sundström, Chem Rev 104:2021-2071, 2004). However, siphonaxanthin-type light-harvesting complexes of photosystem II (LHCII) in siphonous green alga have received fewer studies. In the present study, we determined sequences of genes for several Bryopsis corticulans Lhcbm proteins, which showed that they belong to the group of major LHCII and diverged early from green algae and higher plants. Analysis of pigment composition indicated that this siphonaxanthin-type LHCII contained in total 3 siphonaxanthin and siphonein but no lutein and violaxanthin. In addition, 2 chlorophylls a in higher plant LHCII were replaced by chlorophyll b. These changes led to an increased absorption in green and blue-green light region compared with higher plant LHCII. The binding sites for chlorophylls, siphonaxanthin, and siphonein were suggested based on the structural comparison with that of higher plant LHCII. All of the ligands for the chlorophylls were completely conserved, suggesting that the two chlorophylls b were replaced by chlorophyll a without changing their binding sites in higher plant LHCII. Comparisons of the absorption spectra of isolated siphonaxanthin and siphonein in different organic solutions and the effect of heat treatment suggested that these pigments existed in a low hydrophobic protein environment, leading to an enhancement of light harvesting in the green light region. This low hydrophobic protein environment was maintained by the presence of more serine and threonine residues in B. corticulans LHCII. Finally, esterization of siphonein may also contribute to the enhanced harvesting of green light.

Original languageEnglish
Pages (from-to)267-279
Number of pages13
JournalPhotosynthesis Research
Volume117
Issue number1-3
DOIs
Publication statusPublished - Nov 2013

Fingerprint

Bryopsis
Photosystem II Protein Complex
light harvesting complex
photosystem II
Light
chlorophyll
Methyl Green
Chlorophyta
binding sites
Chlorophyll
Algae
proteins
Pigments
pigments
Proteins
violaxanthin
Binding Sites
siphonaxanthin
Lutein
lutein

Keywords

  • Bryopsis corticulans
  • Carotenoids
  • LHCII
  • Light-harvesting proteins
  • Pigments
  • Siphonaxanthin

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Biochemistry

Cite this

Spectral and functional studies on siphonaxanthin-type light-harvesting complex of photosystem II from Bryopsis corticulans. / Wang, Wenda; Qin, Xiaochun; Sang, Min; Chen, Dongqin; Wang, Kebin; Lin, Rongchen; Lu, Congming; Shen, Jian-Ren; Kuang, Tingyun.

In: Photosynthesis Research, Vol. 117, No. 1-3, 11.2013, p. 267-279.

Research output: Contribution to journalArticle

Wang, Wenda ; Qin, Xiaochun ; Sang, Min ; Chen, Dongqin ; Wang, Kebin ; Lin, Rongchen ; Lu, Congming ; Shen, Jian-Ren ; Kuang, Tingyun. / Spectral and functional studies on siphonaxanthin-type light-harvesting complex of photosystem II from Bryopsis corticulans. In: Photosynthesis Research. 2013 ; Vol. 117, No. 1-3. pp. 267-279.
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AU - Chen, Dongqin

AU - Wang, Kebin

AU - Lin, Rongchen

AU - Lu, Congming

AU - Shen, Jian-Ren

AU - Kuang, Tingyun

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