Specificity of polymerase chain reaction-based clonality analysis of immunoglobulin heavy chain gene rearrangement for the detection of bone marrow infiltrate in B-cell lymphoma-associated haemophagocytic syndrome

Kensuke Kojima, Kinuyo Kaneda, Masaki Yasukawa, Kazuo Tanaka, Takeshi Inoue, Takuya Yamashita, Hiromichi Dansako, Sumie Takase Sakugawa, Teruhiko Kozuka, Masamichi Hara, Mitsune Tanimoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

As a wide range of disorders underlie haemophagocytic syndrome, a rapid distinction between benign polyclonal and malignant monoclonal lymphoid proliferations is critical. We investigated whether polymerase chain reaction (PCR) amplification of immunoglobulin heavy chain gene rearrangement could efficiently detect clonal B-cell populations in non-diagnostic marrow for B-cell lymphoma-associated haemophagocytic syndrome (B-LAHS). On amplifying two DNA samples per biopsy, no reproducible monoclonal PCR result was found in reactive haemophagocytic marrows. In contrast, four out of nine assessable B-LAHS patients with histomorphologically and immunohistochemically lymphoma-free bone marrow showed a reproducible monoclonal immunoglobulin heavy chain gene rearrangement. At the molecular level, two B-LAHS patients had lymphoma-free marrow as demonstrated by patient-specific PCR, suggesting that haemophagocytic marrow is not always associated with lymphoma involvement. PCR-based demonstration of clonal B-cell populations in marrow would add an extra dimension to B-LAHS diagnosis.

Original languageEnglish
Pages (from-to)616-621
Number of pages6
JournalBritish Journal of Haematology
Volume119
Issue number3
DOIs
Publication statusPublished - 2002

Keywords

  • B-cell lymphoma-associated haemophagocytic syndrome
  • Diffuse large B-cell lymphoma
  • Immunoglobulin heavy chain gene rearrangement
  • Intravascular lymphoma
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Hematology

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