Specificity of a heme-assimilating system of Vibrio vulnificus to synthetic heme compounds

Shin-ichi Miyoshi, Takehito Kamei, Yoko Ota, Chiaki Masunaga, Yuko Izuhara, Ken Ichi Tomochika, Sumio Shinoda, Shigeo Yamamoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Vibrio vulnificus strain L-180, a clinical isolate, can obtain iron from a synthetic heme, iron-tetra(4-sulfonatophenyl)porphyrin (Fe-TPPS), as well as from a natural heme, protoheme. This assimilation of iron bound to TPPS was demonstrated to be a common property of V. vulnificus by testing a total of 27 strains isolated from both clinical and environmental sources. Strain L-180 could also utilize Fe-TCPP, but not Fe-TMPyP, as a sole iron source. TPPS or its complex with a metal ion reduced bacterial multiplication in the broth containing a minimum dose of Fe-TPPS. When inoculated into human serum supplemented with Fe-TCPP, L-180 could grow only in the presence of a protease from the same bacterium. In both TPPS and TCPP, each side chain of a porphyrin ring has a negative charge. Therefore, this negative charge may be important for interaction with an outer membrane receptor involving in a heme-assimilating system of V. vulnificus.

Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalFEMS Microbiology Letters
Volume208
Issue number1
DOIs
Publication statusPublished - Feb 19 2002

Fingerprint

Vibrio vulnificus
Heme
Iron
Porphyrins
Peptide Hydrolases
Metals
tetraphenylporphine sulfonate
Ions
Bacteria
Membranes
Serum

Keywords

  • Heme
  • Iron assimilation
  • Metalloporphyrin
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Specificity of a heme-assimilating system of Vibrio vulnificus to synthetic heme compounds. / Miyoshi, Shin-ichi; Kamei, Takehito; Ota, Yoko; Masunaga, Chiaki; Izuhara, Yuko; Tomochika, Ken Ichi; Shinoda, Sumio; Yamamoto, Shigeo.

In: FEMS Microbiology Letters, Vol. 208, No. 1, 19.02.2002, p. 77-81.

Research output: Contribution to journalArticle

Miyoshi, S, Kamei, T, Ota, Y, Masunaga, C, Izuhara, Y, Tomochika, KI, Shinoda, S & Yamamoto, S 2002, 'Specificity of a heme-assimilating system of Vibrio vulnificus to synthetic heme compounds', FEMS Microbiology Letters, vol. 208, no. 1, pp. 77-81. https://doi.org/10.1016/S0378-1097(02)00448-2
Miyoshi, Shin-ichi ; Kamei, Takehito ; Ota, Yoko ; Masunaga, Chiaki ; Izuhara, Yuko ; Tomochika, Ken Ichi ; Shinoda, Sumio ; Yamamoto, Shigeo. / Specificity of a heme-assimilating system of Vibrio vulnificus to synthetic heme compounds. In: FEMS Microbiology Letters. 2002 ; Vol. 208, No. 1. pp. 77-81.
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