Specificity of a heme-assimilating system of Vibrio vulnificus to synthetic heme compounds

Shin-ichi Miyoshi, Takehito Kamei, Yoko Ota, Chiaki Masunaga, Yuko Izuhara, Ken Ichi Tomochika, Sumio Shinoda, Shigeo Yamamoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Vibrio vulnificus strain L-180, a clinical isolate, can obtain iron from a synthetic heme, iron-tetra(4-sulfonatophenyl)porphyrin (Fe-TPPS), as well as from a natural heme, protoheme. This assimilation of iron bound to TPPS was demonstrated to be a common property of V. vulnificus by testing a total of 27 strains isolated from both clinical and environmental sources. Strain L-180 could also utilize Fe-TCPP, but not Fe-TMPyP, as a sole iron source. TPPS or its complex with a metal ion reduced bacterial multiplication in the broth containing a minimum dose of Fe-TPPS. When inoculated into human serum supplemented with Fe-TCPP, L-180 could grow only in the presence of a protease from the same bacterium. In both TPPS and TCPP, each side chain of a porphyrin ring has a negative charge. Therefore, this negative charge may be important for interaction with an outer membrane receptor involving in a heme-assimilating system of V. vulnificus.

Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalFEMS Microbiology Letters
Volume208
Issue number1
DOIs
Publication statusPublished - Feb 19 2002

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Keywords

  • Heme
  • Iron assimilation
  • Metalloporphyrin
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Miyoshi, S., Kamei, T., Ota, Y., Masunaga, C., Izuhara, Y., Tomochika, K. I., Shinoda, S., & Yamamoto, S. (2002). Specificity of a heme-assimilating system of Vibrio vulnificus to synthetic heme compounds. FEMS Microbiology Letters, 208(1), 77-81. https://doi.org/10.1016/S0378-1097(02)00448-2