Specific removal of monocytes from peripheral blood of septic patients by polymyxin B-immobilized filter column

Masahiro Nishibori, Hideo K. Takahashi, Hiroshi Katayama, Shuji Mori, Shinya Saito, Hiromi Iwagaki, Noriaki Tanakac, Kiyoshi Morita, Aiji Ohtsuka

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Lipopolysaccharide (LPS) is one of the major causes of septic shock. The polymyxin B-immobilized filter column (PMX) was developed for the adsorption of endotoxin by direct hemoperfusion and has been used for the treatment of LPS-induced septic shock. In this study, we demonstrated that PMX also specifically bound monocytes from the peripheral blood leukocytes of septic patients by mean of an analysis of bound cells using immunocytochemical and electron microscopic techniques. The specific removal of monocytes from septic patients may produce beneficial effects by reducing the interaction between monocytes and functionally associated cells including vascular endothelial cells.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalActa Medica Okayama
Volume63
Issue number1
Publication statusPublished - Feb 2009

Fingerprint

Polymyxin B
Lipopolysaccharides
Monocytes
Blood
Septic Shock
Endothelial cells
Endotoxins
Hemoperfusion
Adsorption
Electrons
Leukocytes
Endothelial Cells
Therapeutics

Keywords

  • Adsorptive removal
  • Monocyte
  • Polymixin B-immobilized column
  • Septic shock

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Specific removal of monocytes from peripheral blood of septic patients by polymyxin B-immobilized filter column. / Nishibori, Masahiro; Takahashi, Hideo K.; Katayama, Hiroshi; Mori, Shuji; Saito, Shinya; Iwagaki, Hiromi; Tanakac, Noriaki; Morita, Kiyoshi; Ohtsuka, Aiji.

In: Acta Medica Okayama, Vol. 63, No. 1, 02.2009, p. 65-69.

Research output: Contribution to journalArticle

Nishibori, M, Takahashi, HK, Katayama, H, Mori, S, Saito, S, Iwagaki, H, Tanakac, N, Morita, K & Ohtsuka, A 2009, 'Specific removal of monocytes from peripheral blood of septic patients by polymyxin B-immobilized filter column', Acta Medica Okayama, vol. 63, no. 1, pp. 65-69.
Nishibori, Masahiro ; Takahashi, Hideo K. ; Katayama, Hiroshi ; Mori, Shuji ; Saito, Shinya ; Iwagaki, Hiromi ; Tanakac, Noriaki ; Morita, Kiyoshi ; Ohtsuka, Aiji. / Specific removal of monocytes from peripheral blood of septic patients by polymyxin B-immobilized filter column. In: Acta Medica Okayama. 2009 ; Vol. 63, No. 1. pp. 65-69.
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