Specific in vivo labeling with GFP retroviruses, lentiviruses, and adenoviruses for imaging

Robert M. Hoffman, Hiroyuki Kishimoto, Toshiyoshi Fujiwara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fluorescent proteins have revolutionized the field of imaging. Our laboratory pioneered in vivo imaging with fluorescent proteins. Fluorescent proteins have enabled imaging at the subcellular level in mice. We review here the use of different vectors carrying fluorescent proteins to selectively label normal and tumor tissue in vivo. We show that a GFP retrovirus and telomerase-driven GFP adenovirus can selectively label tumors in mice. We also show that a GFP lentivirus can selectively label the liver in mice. The practical application of these results are discussed.

Original languageEnglish
Title of host publicationSmall Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes
DOIs
Publication statusPublished - 2008
EventSmall Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes - San Jose, CA, United States
Duration: Jan 21 2008Jan 22 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6868
ISSN (Print)1605-7422

Other

OtherSmall Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes
CountryUnited States
CitySan Jose, CA
Period1/21/081/22/08

Keywords

  • Cancer
  • Green fluorescent protein
  • Liver
  • Mouse models
  • Selective labeling

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Hoffman, R. M., Kishimoto, H., & Fujiwara, T. (2008). Specific in vivo labeling with GFP retroviruses, lentiviruses, and adenoviruses for imaging. In Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes [68680F] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6868). https://doi.org/10.1117/12.773308