Species-specific B-cell epitope on the C-terminal region of the α antigen from Mycobacterium scrofulaceum

Mikiko Takano, Naoya Ohara, Mariko Naito, Sohkichi Matsumoto, Takemitsu Matsuo, Ryo Shirai, Akio Mizuno, Takeshi Yamada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the amino acid (AA) sequences of α antigen from mycobacteria, C- terminal regions were variable among a variety of mycobacterial species though the N-terminal regions were relatively conserved. These regions may possess some species-specific antigenic determinants of the α antigen from Mycobacterium scrofulaceum (S-α). AAs288-300 of S-α fused to β- galactosidase was reactive with the antisera raised against S-α. The same fused peptide did not react with the antisera raised against the α antigen from Mycobacterium avium (A-α) and Mycobacterium bovis BCG (B-α). B-cell epitope mapping then was performed focusing on the C-terminal region of S-α using the synthetic peptides. Their reactivities with antisera raised against the α antigens of three different mycobacterial species were assessed by ELISA. AAs279-286 were a cross-reactive common immunodominant region among three mycobacterial species. This region may be one of the cross-reactive common epitopes in mycobacterial species. And AAs291-300 were reactive only with the antisera raised against S-α. This region may possess a species- specific epitope.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalMicrobial Pathogenesis
Volume23
Issue number2
DOIs
Publication statusPublished - Aug 1997
Externally publishedYes

Keywords

  • B-cell epitope
  • Mycobacteria
  • Synthetic peptides
  • α antigen

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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