Abstract
In the amino acid (AA) sequences of α antigen from mycobacteria, C- terminal regions were variable among a variety of mycobacterial species though the N-terminal regions were relatively conserved. These regions may possess some species-specific antigenic determinants of the α antigen from Mycobacterium scrofulaceum (S-α). AAs288-300 of S-α fused to β- galactosidase was reactive with the antisera raised against S-α. The same fused peptide did not react with the antisera raised against the α antigen from Mycobacterium avium (A-α) and Mycobacterium bovis BCG (B-α). B-cell epitope mapping then was performed focusing on the C-terminal region of S-α using the synthetic peptides. Their reactivities with antisera raised against the α antigens of three different mycobacterial species were assessed by ELISA. AAs279-286 were a cross-reactive common immunodominant region among three mycobacterial species. This region may be one of the cross-reactive common epitopes in mycobacterial species. And AAs291-300 were reactive only with the antisera raised against S-α. This region may possess a species- specific epitope.
| Original language | English |
|---|---|
| Pages (from-to) | 95-100 |
| Number of pages | 6 |
| Journal | Microbial Pathogenesis |
| Volume | 23 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 1997 |
| Externally published | Yes |
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Keywords
- α antigen
- B-cell epitope
- Mycobacteria
- Synthetic peptides
ASJC Scopus subject areas
- Microbiology
- Infectious Diseases
Cite this
Species-specific B-cell epitope on the C-terminal region of the α antigen from Mycobacterium scrofulaceum. / Takano, Mikiko; Oohara, Naoya; Naito, Mariko; Matsumoto, Sohkichi; Matsuo, Takemitsu; Shirai, Ryo; Mizuno, Akio; Yamada, Takeshi.
In: Microbial Pathogenesis, Vol. 23, No. 2, 08.1997, p. 95-100.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Species-specific B-cell epitope on the C-terminal region of the α antigen from Mycobacterium scrofulaceum
AU - Takano, Mikiko
AU - Oohara, Naoya
AU - Naito, Mariko
AU - Matsumoto, Sohkichi
AU - Matsuo, Takemitsu
AU - Shirai, Ryo
AU - Mizuno, Akio
AU - Yamada, Takeshi
PY - 1997/8
Y1 - 1997/8
N2 - In the amino acid (AA) sequences of α antigen from mycobacteria, C- terminal regions were variable among a variety of mycobacterial species though the N-terminal regions were relatively conserved. These regions may possess some species-specific antigenic determinants of the α antigen from Mycobacterium scrofulaceum (S-α). AAs288-300 of S-α fused to β- galactosidase was reactive with the antisera raised against S-α. The same fused peptide did not react with the antisera raised against the α antigen from Mycobacterium avium (A-α) and Mycobacterium bovis BCG (B-α). B-cell epitope mapping then was performed focusing on the C-terminal region of S-α using the synthetic peptides. Their reactivities with antisera raised against the α antigens of three different mycobacterial species were assessed by ELISA. AAs279-286 were a cross-reactive common immunodominant region among three mycobacterial species. This region may be one of the cross-reactive common epitopes in mycobacterial species. And AAs291-300 were reactive only with the antisera raised against S-α. This region may possess a species- specific epitope.
AB - In the amino acid (AA) sequences of α antigen from mycobacteria, C- terminal regions were variable among a variety of mycobacterial species though the N-terminal regions were relatively conserved. These regions may possess some species-specific antigenic determinants of the α antigen from Mycobacterium scrofulaceum (S-α). AAs288-300 of S-α fused to β- galactosidase was reactive with the antisera raised against S-α. The same fused peptide did not react with the antisera raised against the α antigen from Mycobacterium avium (A-α) and Mycobacterium bovis BCG (B-α). B-cell epitope mapping then was performed focusing on the C-terminal region of S-α using the synthetic peptides. Their reactivities with antisera raised against the α antigens of three different mycobacterial species were assessed by ELISA. AAs279-286 were a cross-reactive common immunodominant region among three mycobacterial species. This region may be one of the cross-reactive common epitopes in mycobacterial species. And AAs291-300 were reactive only with the antisera raised against S-α. This region may possess a species- specific epitope.
KW - α antigen
KW - B-cell epitope
KW - Mycobacteria
KW - Synthetic peptides
UR - http://www.scopus.com/inward/record.url?scp=0030832194&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030832194&partnerID=8YFLogxK
U2 - 10.1006/mpat.1997.0147
DO - 10.1006/mpat.1997.0147
M3 - Article
VL - 23
SP - 95
EP - 100
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
SN - 0882-4010
IS - 2
ER -