Species-specific B-cell epitope on the C-terminal region of the α antigen from Mycobacterium scrofulaceum

Mikiko Takano, Naoya Oohara, Mariko Naito, Sohkichi Matsumoto, Takemitsu Matsuo, Ryo Shirai, Akio Mizuno, Takeshi Yamada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the amino acid (AA) sequences of α antigen from mycobacteria, C- terminal regions were variable among a variety of mycobacterial species though the N-terminal regions were relatively conserved. These regions may possess some species-specific antigenic determinants of the α antigen from Mycobacterium scrofulaceum (S-α). AAs288-300 of S-α fused to β- galactosidase was reactive with the antisera raised against S-α. The same fused peptide did not react with the antisera raised against the α antigen from Mycobacterium avium (A-α) and Mycobacterium bovis BCG (B-α). B-cell epitope mapping then was performed focusing on the C-terminal region of S-α using the synthetic peptides. Their reactivities with antisera raised against the α antigens of three different mycobacterial species were assessed by ELISA. AAs279-286 were a cross-reactive common immunodominant region among three mycobacterial species. This region may be one of the cross-reactive common epitopes in mycobacterial species. And AAs291-300 were reactive only with the antisera raised against S-α. This region may possess a species- specific epitope.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalMicrobial Pathogenesis
Volume23
Issue number2
DOIs
Publication statusPublished - Aug 1997
Externally publishedYes

Fingerprint

Mycobacterium scrofulaceum
B-Lymphocyte Epitopes
Immune Sera
Antigens
Epitopes
Mycobacterium bovis
Galactosidases
Epitope Mapping
Mycobacterium avium
Immunodominant Epitopes
Peptides
Mycobacterium
Amino Acid Sequence
Enzyme-Linked Immunosorbent Assay

Keywords

  • α antigen
  • B-cell epitope
  • Mycobacteria
  • Synthetic peptides

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Species-specific B-cell epitope on the C-terminal region of the α antigen from Mycobacterium scrofulaceum. / Takano, Mikiko; Oohara, Naoya; Naito, Mariko; Matsumoto, Sohkichi; Matsuo, Takemitsu; Shirai, Ryo; Mizuno, Akio; Yamada, Takeshi.

In: Microbial Pathogenesis, Vol. 23, No. 2, 08.1997, p. 95-100.

Research output: Contribution to journalArticle

Takano, M, Oohara, N, Naito, M, Matsumoto, S, Matsuo, T, Shirai, R, Mizuno, A & Yamada, T 1997, 'Species-specific B-cell epitope on the C-terminal region of the α antigen from Mycobacterium scrofulaceum', Microbial Pathogenesis, vol. 23, no. 2, pp. 95-100. https://doi.org/10.1006/mpat.1997.0147
Takano, Mikiko ; Oohara, Naoya ; Naito, Mariko ; Matsumoto, Sohkichi ; Matsuo, Takemitsu ; Shirai, Ryo ; Mizuno, Akio ; Yamada, Takeshi. / Species-specific B-cell epitope on the C-terminal region of the α antigen from Mycobacterium scrofulaceum. In: Microbial Pathogenesis. 1997 ; Vol. 23, No. 2. pp. 95-100.
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