TY - JOUR
T1 - Species identification of blood and bloodstains by enzyme-linked immunosorbent assay (ELISA) using anti-human immunoglobulin kappa light chain monoclonal antibody
AU - Yamamoto, Yuji
AU - Tsutsumi, Akira
AU - Ishizu, Hideo
N1 - Funding Information:
This study was supported in part by the Grant-in-Aid for Scientific Research (No. 60570276) from the Ministry of Education, Science and Culture, Japan. The main part of this work was presented at the 11th Meeting of the International Association of Forensic Sciences in Vancouver, Canada, August 5,1987.
PY - 1989/1
Y1 - 1989/1
N2 - A series of experiments was conducted to establish a species identification method by means of the enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody with high specificity, the result of which is reported. The limit of human IgG detection by inhibition ELISA using mouse anti-human immunoglobulin kappa light chain (Ig x) monoclonal antibody is 0.01 μg. Using this method it was possible to detect human Ig x from bloodstains in dilutions up to 1: 100 000 (dilution multiple of bloodstain extract to the original amount of blood). Human Ig x was detectable in human bloodstains in dilutions up to 1:50 000 one year after stain preparation and in dilutions up to 1:10 000 4 years after stain preparation, showing that this method is applicable to species identification of old bloodstains. When primates other than human were studied for specificity, red-chested tamarin and Japanese monkey showed no cross reaction at all. Cross reaction was noted with a chimpanzee, but experimentally it could be differentiated from human. Furthermore, no cross reaction was noted with respect to blood samples from non-primates (guinea pig, sheep, horse, dog and cat).
AB - A series of experiments was conducted to establish a species identification method by means of the enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody with high specificity, the result of which is reported. The limit of human IgG detection by inhibition ELISA using mouse anti-human immunoglobulin kappa light chain (Ig x) monoclonal antibody is 0.01 μg. Using this method it was possible to detect human Ig x from bloodstains in dilutions up to 1: 100 000 (dilution multiple of bloodstain extract to the original amount of blood). Human Ig x was detectable in human bloodstains in dilutions up to 1:50 000 one year after stain preparation and in dilutions up to 1:10 000 4 years after stain preparation, showing that this method is applicable to species identification of old bloodstains. When primates other than human were studied for specificity, red-chested tamarin and Japanese monkey showed no cross reaction at all. Cross reaction was noted with a chimpanzee, but experimentally it could be differentiated from human. Furthermore, no cross reaction was noted with respect to blood samples from non-primates (guinea pig, sheep, horse, dog and cat).
KW - Blood
KW - ELISA
KW - Monoclonal anti-human immunoglobulin kappa light chain
KW - Species identification
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U2 - 10.1016/0379-0738(89)90171-0
DO - 10.1016/0379-0738(89)90171-0
M3 - Article
C2 - 2494103
AN - SCOPUS:0024498082
VL - 40
SP - 85
EP - 95
JO - Forensic Science International
JF - Forensic Science International
SN - 0379-0738
IS - 1
ER -