Spatiotemporal expression of four isoforms of Ca2+/calmodulin-dependent protein kinase I in brain and its possible roles in hippocampal dendritic growth

Akifumi Kamata, Hiroyuki Sakagami, Hiroshi Tokumitsu, Yuji Owada, Kohji Fukunaga, Hisatake Kondo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Among multifunctional Ca2+/calmodulin-dependent protein kinases (CaMKs), CaMKI has been shown to comprise a family of four structurally related isoforms (α, β, γ, and δ) encoded by separate genes with abundant expression in mature brain. In this study, we first examined the developmental gene expression of the four isoforms of CaMKI in mouse brain with special attention to the hippocampal formation by in situ hybridization analysis. The four isoforms of CaMKI were found to exhibit distinct spatiotemporal expression during neuronal development. We also examined the functional involvement of CaMKI in the dendritic formation of cultured hippocampal neurons. The overexpression of kinase-dead mutants of CaMKI reduced the average dendritic length of the transfected neurons without any significant effects on the number of primary dendrites and the branching index. Our present findings provide the detailed anatomical information on the developmental expression of the four isoforms of CaMKI in mouse brain, which represents the possible functional involvement of CaMKI in the basal dendritic growth of hippocampal neurons.

Original languageEnglish
Pages (from-to)86-97
Number of pages12
JournalNeuroscience Research
Volume57
Issue number1
DOIs
Publication statusPublished - Jan 2007
Externally publishedYes

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Type 1
Protein Isoforms
Brain
Growth
Neurons
Hippocampus
Developmental Genes
Calcium-Calmodulin-Dependent Protein Kinases
Dendrites
In Situ Hybridization
Phosphotransferases
Gene Expression
Genes

Keywords

  • Calcium
  • Calmodulin
  • Dendrite
  • Hippocampus
  • Kinase
  • Plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Spatiotemporal expression of four isoforms of Ca2+/calmodulin-dependent protein kinase I in brain and its possible roles in hippocampal dendritic growth. / Kamata, Akifumi; Sakagami, Hiroyuki; Tokumitsu, Hiroshi; Owada, Yuji; Fukunaga, Kohji; Kondo, Hisatake.

In: Neuroscience Research, Vol. 57, No. 1, 01.2007, p. 86-97.

Research output: Contribution to journalArticle

Kamata, Akifumi ; Sakagami, Hiroyuki ; Tokumitsu, Hiroshi ; Owada, Yuji ; Fukunaga, Kohji ; Kondo, Hisatake. / Spatiotemporal expression of four isoforms of Ca2+/calmodulin-dependent protein kinase I in brain and its possible roles in hippocampal dendritic growth. In: Neuroscience Research. 2007 ; Vol. 57, No. 1. pp. 86-97.
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