Spatiotemporal changes of free cholesterol and neutral lipids after transient focal cerebral ischemia in rats

H. Kamada, K. Sato, M. Iwai, K. Ohta, W. R. Zhang, I. Nagano, M. Shoji, Koji Abe

Research output: Contribution to journalArticle

Abstract

We investigated temporal and spatial changes of free cholesterol (FC) and neutral lipids (NLs) after brain ischemia with filipin complex staining to detect mainly FC and Nile Red staining for NLs such as cholesteryl ester (CE) and triacylglyceride (TAG). Filipin stanining decreased during 1–7 days and lost at 21 days after transient middle cerebral artery (MCA) occlusion in the ischemic core, but did not change in the penumbra. Nile Red positive droplets reached the maximum at 7 days after transient MCA occlusion (MCAO) and gradually decreased in the core, while the peak time delayed in the penumbra. Most Nile Red positive droplets were double positive for ED1 in the core, and were localized within GFAP positive cells in the penumbra. The present study suggests that changes of FC and NLs are different temporally and spatially between the ischemic core and the penumbra in relation to degenerative and regenerative neural processes following brain ischemia. Macrophages and astrocytes are presumed to play important roles in lipid metabolism for neural reorganization of the ischemic brain injury in the ischemic core and the penumbra, respectively.

Original languageEnglish
Pages (from-to)163-169
Number of pages7
JournalInternational Congress Series
Volume1252
Issue numberC
DOIs
Publication statusPublished - Jun 1 2003

Fingerprint

Transient Ischemic Attack
Filipin
Middle Cerebral Artery Infarction
Cholesterol
Brain Ischemia
Lipids
Staining and Labeling
Cholesterol Esters
Lipid Metabolism
Astrocytes
Brain Injuries
Macrophages
nile red

Keywords

  • Brain ischemia
  • Cholesterol
  • Lipid
  • Rat

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Spatiotemporal changes of free cholesterol and neutral lipids after transient focal cerebral ischemia in rats. / Kamada, H.; Sato, K.; Iwai, M.; Ohta, K.; Zhang, W. R.; Nagano, I.; Shoji, M.; Abe, Koji.

In: International Congress Series, Vol. 1252, No. C, 01.06.2003, p. 163-169.

Research output: Contribution to journalArticle

Kamada, H. ; Sato, K. ; Iwai, M. ; Ohta, K. ; Zhang, W. R. ; Nagano, I. ; Shoji, M. ; Abe, Koji. / Spatiotemporal changes of free cholesterol and neutral lipids after transient focal cerebral ischemia in rats. In: International Congress Series. 2003 ; Vol. 1252, No. C. pp. 163-169.
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