Spatiotemporal analysis of caveolae dynamics using total internal reflection fluorescence microscopy

Yosuke Senju, Shiro Suetsugu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Total internal reflection fluorescence microscopy enables to analyze the localizations and dynamics of cellular events that occur at or near the plasma membrane. Total internal reflection fluorescence microscopy exclusively illuminates molecules in the close vicinity of the glass surface, thereby reducing background fluorescence and enabling observation of the plasma membrane in the glass-attached cells with a high signal-to-noise ratio. Here, we describe the application of total internal reflection fluorescence microscopy to analyze the dynamics of caveolae, which play essential physiological functions, including membrane tension buffering, endocytosis, and signaling at the plasma membrane.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages63-70
Number of pages8
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2169
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Caveolae
  • Endocytosis
  • Kymograph
  • Lateral diffusion
  • Total internal reflection fluorescence microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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