Spatially and temporally different expression of osteonectin and osteopontin in the infarct zone of experimentally induced myocardial infarction in rats

Issei Komatsubara, Takashi Murakami, Shozo Kusachi, Keigo Nakamura, Satoshi Hirohata, Junichi Hayashi, Shunji Takemoto, Chisato Suezawa, Yoshifumi Ninomiya, Yasushi Shiratori

Research output: Contribution to journalArticle

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Abstract

Osteonectin and osteopontin, two secreted matricellular proteins, have a variety of functions that are exerted through interaction with matrix components. These proteins appear in response to tissue injury. To test our hypothesis that osteopontin and osteonectin are expressed with spatially and temporally different patterns in myocardial infarct tissue, we investigated osteonectin and osteopontin expression in experimentally induced myocardial infarction in rats, in comparison with Type I collagen expression. Northern blotting demonstrated that osteonectin mRNA did not markedly increase on Day 2 after the infarction, but it increased on Days 7 and 14 by 1.7±0.12- and 1.8±0.01-fold compared to that in preligation hearts. In contrast, osteopontin mRNA was increased on Day 1 (41.9±11.3-fold increase) and on Day 2 (58.3±7.6-fold increase), and then it declined on Days 7 and 14 (24.8±9.0- and 13.5±4.7-fold increase, respectively). In situ hybridization revealed that osteonectin mRNA signals were observed in fibroblasts, myofibroblasts and macrophages around infarct necrotic tissue on Days 7 and 14. Osteopontin mRNA signals were observed in macrophages in the infarct marginal zone on Day 2. Immunopositive staining for both osteonectin and osteopontin showed the same pattern as that obtained by in situ hybridization. The time course of osteonectin mRNA was almost parallel with that of Type I collagen mRNA, while that of osteopontin was not. These results demonstrated spatially and temporally different expression patterns of osteonectin and osteopontin in myocardial infarction and suggest that osteonectin appears to be involved in the pathological course in the late phase after infarction concomitantly with Type I collagen, while osteopontin may play a role in the early phase.

Original languageEnglish
Pages (from-to)186-194
Number of pages9
JournalCardiovascular Pathology
Volume12
Issue number4
DOIs
Publication statusPublished - Jul 2003

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Osteonectin
Osteopontin
Myocardial Infarction
Messenger RNA
Collagen Type I
Infarction
In Situ Hybridization
Macrophages
Myofibroblasts
Northern Blotting
Proteins
Fibroblasts
Staining and Labeling

Keywords

  • Immunohistochemistry
  • Ischemic heart disease
  • Matricellular proteins
  • Molecular biology
  • Myocardial remodeling

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pathology and Forensic Medicine

Cite this

Spatially and temporally different expression of osteonectin and osteopontin in the infarct zone of experimentally induced myocardial infarction in rats. / Komatsubara, Issei; Murakami, Takashi; Kusachi, Shozo; Nakamura, Keigo; Hirohata, Satoshi; Hayashi, Junichi; Takemoto, Shunji; Suezawa, Chisato; Ninomiya, Yoshifumi; Shiratori, Yasushi.

In: Cardiovascular Pathology, Vol. 12, No. 4, 07.2003, p. 186-194.

Research output: Contribution to journalArticle

Komatsubara, I, Murakami, T, Kusachi, S, Nakamura, K, Hirohata, S, Hayashi, J, Takemoto, S, Suezawa, C, Ninomiya, Y & Shiratori, Y 2003, 'Spatially and temporally different expression of osteonectin and osteopontin in the infarct zone of experimentally induced myocardial infarction in rats', Cardiovascular Pathology, vol. 12, no. 4, pp. 186-194. https://doi.org/10.1016/S1054-8807(03)00042-5
Komatsubara, Issei ; Murakami, Takashi ; Kusachi, Shozo ; Nakamura, Keigo ; Hirohata, Satoshi ; Hayashi, Junichi ; Takemoto, Shunji ; Suezawa, Chisato ; Ninomiya, Yoshifumi ; Shiratori, Yasushi. / Spatially and temporally different expression of osteonectin and osteopontin in the infarct zone of experimentally induced myocardial infarction in rats. In: Cardiovascular Pathology. 2003 ; Vol. 12, No. 4. pp. 186-194.
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AU - Hirohata, Satoshi

AU - Hayashi, Junichi

AU - Takemoto, Shunji

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