Spatial regulation of specific gene expression through photoactivation of RNAi

Tamaki Endoh; Masahiko Sisido; Takashi Ohtsuki

doi:10.1016/j.jconrel.2009.04.015

Spatial regulation of specific gene expression through photoactivation of RNAi

Tamaki Endoh, Masahiko Sisido, Takashi Ohtsuki

Research output: Contribution to journal › Article

32 Citations (Scopus)

Abstract

In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

Original language	English
Pages (from-to)	241-245
Number of pages	5
Journal	Journal of Controlled Release
Volume	137
Issue number	3
DOIs	https://doi.org/10.1016/j.jconrel.2009.04.015
Publication status	Published - Aug 4 2009

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Keywords

Photoregulation
RNA carrier
RNAi
shRNA delivery
TatU1A

ASJC Scopus subject areas

Pharmaceutical Science

Cite this

Endoh, T., Sisido, M., & Ohtsuki, T. (2009). Spatial regulation of specific gene expression through photoactivation of RNAi. Journal of Controlled Release, 137(3), 241-245. https://doi.org/10.1016/j.jconrel.2009.04.015

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Access to Document

10.1016/j.jconrel.2009.04.015