Spatial regulation of specific gene expression through photoactivation of RNAi

Tamaki Endoh; Masahiko Sisido; Takashi Ohtsuki

doi:10.1016/j.jconrel.2009.04.015

Spatial regulation of specific gene expression through photoactivation of RNAi

Tamaki Endoh, Masahiko Sisido, Takashi Ohtsuki

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

Original language	English
Pages (from-to)	241-245
Number of pages	5
Journal	Journal of Controlled Release
Volume	137
Issue number	3
DOIs	https://doi.org/10.1016/j.jconrel.2009.04.015
Publication status	Published - Aug 4 2009

Keywords

Photoregulation
RNA carrier
RNAi
TatU1A
shRNA delivery

ASJC Scopus subject areas

Pharmaceutical Science

Access to Document

10.1016/j.jconrel.2009.04.015

Cite this

@article{8f676336713a4fafbb32e0cf0d851e29,

title = "Spatial regulation of specific gene expression through photoactivation of RNAi",

abstract = "In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.",

keywords = "Photoregulation, RNA carrier, RNAi, TatU1A, shRNA delivery",

author = "Tamaki Endoh and Masahiko Sisido and Takashi Ohtsuki",

note = "Funding Information: We thank Dr. Y. Okamoto (Okayama Univ.) for the optical measuring instrument. This work was supported by a grant from NEDO (05A02707a) and Grant-in-Aid for Scientific Research of Priority Areas: Cancer to T. O. ",

year = "2009",

month = aug,

day = "4",

doi = "10.1016/j.jconrel.2009.04.015",

language = "English",

volume = "137",

pages = "241--245",

journal = "Journal of Controlled Release",

issn = "0168-3659",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Spatial regulation of specific gene expression through photoactivation of RNAi

AU - Endoh, Tamaki

AU - Sisido, Masahiko

AU - Ohtsuki, Takashi

N1 - Funding Information: We thank Dr. Y. Okamoto (Okayama Univ.) for the optical measuring instrument. This work was supported by a grant from NEDO (05A02707a) and Grant-in-Aid for Scientific Research of Priority Areas: Cancer to T. O.

PY - 2009/8/4

Y1 - 2009/8/4

N2 - In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

AB - In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

KW - Photoregulation

KW - RNA carrier

KW - RNAi

KW - TatU1A

KW - shRNA delivery

UR - http://www.scopus.com/inward/record.url?scp=67649117861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649117861&partnerID=8YFLogxK

U2 - 10.1016/j.jconrel.2009.04.015

DO - 10.1016/j.jconrel.2009.04.015

M3 - Article

C2 - 19376174

AN - SCOPUS:67649117861

VL - 137

SP - 241

EP - 245

JO - Journal of Controlled Release

JF - Journal of Controlled Release

SN - 0168-3659

IS - 3

ER -

Spatial regulation of specific gene expression through photoactivation of RNAi

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this