Abstract
In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.
| Original language | English |
|---|---|
| Pages (from-to) | 241-245 |
| Number of pages | 5 |
| Journal | Journal of Controlled Release |
| Volume | 137 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Aug 4 2009 |
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Keywords
- Photoregulation
- RNA carrier
- RNAi
- shRNA delivery
- TatU1A
ASJC Scopus subject areas
- Pharmaceutical Science
Cite this
Spatial regulation of specific gene expression through photoactivation of RNAi. / Endoh, Tamaki; Sisido, Masahiko; Ohtsuki, Takashi.
In: Journal of Controlled Release, Vol. 137, No. 3, 04.08.2009, p. 241-245.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Spatial regulation of specific gene expression through photoactivation of RNAi
AU - Endoh, Tamaki
AU - Sisido, Masahiko
AU - Ohtsuki, Takashi
PY - 2009/8/4
Y1 - 2009/8/4
N2 - In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.
AB - In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.
KW - Photoregulation
KW - RNA carrier
KW - RNAi
KW - shRNA delivery
KW - TatU1A
UR - http://www.scopus.com/inward/record.url?scp=67649117861&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67649117861&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2009.04.015
DO - 10.1016/j.jconrel.2009.04.015
M3 - Article
C2 - 19376174
AN - SCOPUS:67649117861
VL - 137
SP - 241
EP - 245
JO - Journal of Controlled Release
JF - Journal of Controlled Release
SN - 0168-3659
IS - 3
ER -