Spatial regulation of specific gene expression through photoactivation of RNAi

Tamaki Endoh; Masahiko Sisido; Takashi Ohtsuki

doi:10.1016/j.jconrel.2009.04.015

Spatial regulation of specific gene expression through photoactivation of RNAi

Tamaki Endoh, Masahiko Sisido, Takashi Ohtsuki

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

Original language	English
Pages (from-to)	241-245
Number of pages	5
Journal	Journal of Controlled Release
Volume	137
Issue number	3
DOIs	https://doi.org/10.1016/j.jconrel.2009.04.015
Publication status	Published - Aug 4 2009

Keywords

Photoregulation
RNA carrier
RNAi
TatU1A
shRNA delivery

ASJC Scopus subject areas

Pharmaceutical Science

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10.1016/j.jconrel.2009.04.015

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title = "Spatial regulation of specific gene expression through photoactivation of RNAi",

abstract = "In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.",

keywords = "Photoregulation, RNA carrier, RNAi, TatU1A, shRNA delivery",

author = "Tamaki Endoh and Masahiko Sisido and Takashi Ohtsuki",

note = "Funding Information: We thank Dr. Y. Okamoto (Okayama Univ.) for the optical measuring instrument. This work was supported by a grant from NEDO (05A02707a) and Grant-in-Aid for Scientific Research of Priority Areas: Cancer to T. O. ",

year = "2009",

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doi = "10.1016/j.jconrel.2009.04.015",

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T1 - Spatial regulation of specific gene expression through photoactivation of RNAi

AU - Endoh, Tamaki

AU - Sisido, Masahiko

AU - Ohtsuki, Takashi

N1 - Funding Information: We thank Dr. Y. Okamoto (Okayama Univ.) for the optical measuring instrument. This work was supported by a grant from NEDO (05A02707a) and Grant-in-Aid for Scientific Research of Priority Areas: Cancer to T. O.

PY - 2009/8/4

Y1 - 2009/8/4

N2 - In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

AB - In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

KW - Photoregulation

KW - RNA carrier

KW - RNAi

KW - TatU1A

KW - shRNA delivery

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