Spatial regulation of specific gene expression through photoactivation of RNAi

Tamaki Endoh, Masahiko Sisido, Takashi Ohtsuki

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalJournal of Controlled Release
Volume137
Issue number3
DOIs
Publication statusPublished - Aug 4 2009

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Keywords

  • Photoregulation
  • RNA carrier
  • RNAi
  • shRNA delivery
  • TatU1A

ASJC Scopus subject areas

  • Pharmaceutical Science

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