Spatial regulation of specific gene expression through photoactivation of RNAi

Tamaki Endoh, Masahiko Sisido, Takashi Ohtsuki

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalJournal of Controlled Release
Volume137
Issue number3
DOIs
Publication statusPublished - Aug 4 2009

Fingerprint

Gene Expression Regulation
RNA Interference
Light
Halogens
Gene Silencing
Fluorescent Dyes
Carrier Proteins
Lasers
Coloring Agents
Cell Culture Techniques
RNA

Keywords

  • Photoregulation
  • RNA carrier
  • RNAi
  • shRNA delivery
  • TatU1A

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Spatial regulation of specific gene expression through photoactivation of RNAi. / Endoh, Tamaki; Sisido, Masahiko; Ohtsuki, Takashi.

In: Journal of Controlled Release, Vol. 137, No. 3, 04.08.2009, p. 241-245.

Research output: Contribution to journalArticle

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