Abstract
In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.
| Original language | English |
|---|---|
| Pages (from-to) | 241-245 |
| Number of pages | 5 |
| Journal | Journal of Controlled Release |
| Volume | 137 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Aug 4 2009 |
Keywords
- Photoregulation
- RNA carrier
- RNAi
- TatU1A
- shRNA delivery
ASJC Scopus subject areas
- Pharmaceutical Science
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Endoh, T., Sisido, M., & Ohtsuki, T. (2009). Spatial regulation of specific gene expression through photoactivation of RNAi. Journal of Controlled Release, 137(3), 241-245. https://doi.org/10.1016/j.jconrel.2009.04.015