Abstract
In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.
Original language | English |
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Pages (from-to) | 241-245 |
Number of pages | 5 |
Journal | Journal of Controlled Release |
Volume | 137 |
Issue number | 3 |
DOIs | |
Publication status | Published - Aug 4 2009 |
Keywords
- Photoregulation
- RNA carrier
- RNAi
- TatU1A
- shRNA delivery
ASJC Scopus subject areas
- Pharmaceutical Science