Spatial distribution, diversity and composition of bacterial communities in sub-seafloor fluids at a deep-sea hydrothermal field of the Suiyo Seamount

Shingo Kato, Kurt Hara, Hiroko Kasai, Takashi Teramura, Michinari Sunamura, Jun ichiro Ishibashi, Takeshi Kakegawa, Toshiro Yamanaka, Hiroyuki Kimura, Katsumi Marumo, Tetsuro Urabe, Akihiko Yamagishi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Spatial distribution, diversity, and composition of bacterial communities within the shallow sub-seafloor at the deep-sea hydrothermal field of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific Ocean, were investigated. Fluids were sampled from four boreholes in this area. Each borehole was located near or away from active vents, the distance ranging 2-40 m from active vents. In addition, fluids discharging from a natural vent and ambient seawater were sampled in this area. We extracted DNA from each sample, amplified bacterial 16S rRNA genes by PCR, cloned the PCR products and sequenced. The total number of clones analyzed was 348. Most of the detected phylotypes were affiliated with the phylum Proteobacteria, of which the detection frequency in each clone library ranged from 84.6% to 100%. The bacterial community diversity and composition were different between hydrothermal fluids and seawater, between fluids from the boreholes and the vent, and even among fluids from each borehole. The relative abundances of the phylotypes related to Thiomicrospira, Methylobacterium and Sphingomonas were significantly different among fluids from each borehole. The phylotypes related to Thiomicrospira and Alcanivorax were detected in all of the boreholes and vent samples. Our findings provide insights into bacterial communities in the shallow sub-seafloor environments at active deep-sea hydrothermal vent fields.

Original languageEnglish
Pages (from-to)1844-1855
Number of pages12
JournalDeep-Sea Research Part I: Oceanographic Research Papers
Volume56
Issue number10
DOIs
Publication statusPublished - Oct 1 2009

Keywords

  • 16S ribosomal RNA gene
  • Bacteria
  • Deep-sea hydrothermal vent
  • Izu-Bonin Island Arc
  • Japan
  • Sub-seafloor
  • Suiyo Seamount

ASJC Scopus subject areas

  • Oceanography
  • Aquatic Science

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    Kato, S., Hara, K., Kasai, H., Teramura, T., Sunamura, M., Ishibashi, J. I., Kakegawa, T., Yamanaka, T., Kimura, H., Marumo, K., Urabe, T., & Yamagishi, A. (2009). Spatial distribution, diversity and composition of bacterial communities in sub-seafloor fluids at a deep-sea hydrothermal field of the Suiyo Seamount. Deep-Sea Research Part I: Oceanographic Research Papers, 56(10), 1844-1855. https://doi.org/10.1016/j.dsr.2009.05.004