TY - GEN
T1 - Spatial and temporal application of microfluidics to cells
AU - Yamada, Akira
AU - Katanosaka, Yuki
AU - Mohri, Satoshi
AU - Naruse, Keiji
PY - 2006/12/1
Y1 - 2006/12/1
N2 - The rapid solution exchange around the adhesive single cell has recently received a great deal of attention from the viewpoint cell response analyses. We recently constructed a new system, which enables the replacement of solutions rapidly and precisely, by utilizing laminar flow technology. The sharp boundary of the adjoining two liquids (no mixing) moves around the cells, and minimum increments of the boundary shift are controlled at an interval of roughly 1.6 μm, and the time required for this movement is 20 msec. The system can be used for the analysis of intracellular Ca2+ concentrations using a fluorescent indicator, in which the cells are thus stimulated by the rapid exchange of the solution. These results suggest that the spatial and temporal control of the solution around the adhesive single cell can thus be realized.
AB - The rapid solution exchange around the adhesive single cell has recently received a great deal of attention from the viewpoint cell response analyses. We recently constructed a new system, which enables the replacement of solutions rapidly and precisely, by utilizing laminar flow technology. The sharp boundary of the adjoining two liquids (no mixing) moves around the cells, and minimum increments of the boundary shift are controlled at an interval of roughly 1.6 μm, and the time required for this movement is 20 msec. The system can be used for the analysis of intracellular Ca2+ concentrations using a fluorescent indicator, in which the cells are thus stimulated by the rapid exchange of the solution. These results suggest that the spatial and temporal control of the solution around the adhesive single cell can thus be realized.
UR - http://www.scopus.com/inward/record.url?scp=50449093877&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50449093877&partnerID=8YFLogxK
U2 - 10.1109/MHS.2006.320281
DO - 10.1109/MHS.2006.320281
M3 - Conference contribution
AN - SCOPUS:50449093877
SN - 1424407176
SN - 9781424407170
T3 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
BT - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
T2 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
Y2 - 5 November 2006 through 8 November 2006
ER -