Spatial and temporal application of microfluidics to cells

Akira Yamada, Yuki Katanosaka, Satoshi Mohri, Keiji Naruse

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

The rapid solution exchange around the adhesive single cell has recently received a great deal of attention from the viewpoint cell response analyses. We recently constructed a new system, which enables the replacement of solutions rapidly and precisely, by utilizing laminar flow technology. The sharp boundary of the adjoining two liquids (no mixing) moves around the cells, and minimum increments of the boundary shift are controlled at an interval of roughly 1.6 μm, and the time required for this movement is 20 msec. The system can be used for the analysis of intracellular Ca2+ concentrations using a fluorescent indicator, in which the cells are thus stimulated by the rapid exchange of the solution. These results suggest that the spatial and temporal control of the solution around the adhesive single cell can thus be realized.

Original languageEnglish
Title of host publication2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
DOIs
Publication statusPublished - Dec 1 2006
Event2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS - Nagoya, Japan
Duration: Nov 5 2006Nov 8 2006

Publication series

Name2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS

Other

Other2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
CountryJapan
CityNagoya
Period11/5/0611/8/06

ASJC Scopus subject areas

  • Computational Mechanics
  • Control and Systems Engineering
  • Human Factors and Ergonomics

Fingerprint Dive into the research topics of 'Spatial and temporal application of microfluidics to cells'. Together they form a unique fingerprint.

  • Cite this

    Yamada, A., Katanosaka, Y., Mohri, S., & Naruse, K. (2006). Spatial and temporal application of microfluidics to cells. In 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS [4110387] (2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS). https://doi.org/10.1109/MHS.2006.320281