Spatial and temporal application of microfluidics to cells

Akira Yamada; Yuki Katanosaka; Satoshi Mohri; Keiji Naruse

doi:10.1109/MHS.2006.320281

Spatial and temporal application of microfluidics to cells

Akira Yamada, Yuki Katanosaka, Satoshi Mohri, Keiji Naruse

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

The rapid solution exchange around the adhesive single cell has recently received a great deal of attention from the viewpoint cell response analyses. We recently constructed a new system, which enables the replacement of solutions rapidly and precisely, by utilizing laminar flow technology. The sharp boundary of the adjoining two liquids (no mixing) moves around the cells, and minimum increments of the boundary shift are controlled at an interval of roughly 1.6 μm, and the time required for this movement is 20 msec. The system can be used for the analysis of intracellular Ca²⁺ concentrations using a fluorescent indicator, in which the cells are thus stimulated by the rapid exchange of the solution. These results suggest that the spatial and temporal control of the solution around the adhesive single cell can thus be realized.

Original language	English
Title of host publication	2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
DOIs	https://doi.org/10.1109/MHS.2006.320281
Publication status	Published - Dec 1 2006
Event	2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS - Nagoya, Japan Duration: Nov 5 2006 → Nov 8 2006

Publication series

Name	2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS

Other

Other	2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
Country	Japan
City	Nagoya
Period	11/5/06 → 11/8/06

ASJC Scopus subject areas

Computational Mechanics
Control and Systems Engineering
Human Factors and Ergonomics

Access to Document

10.1109/MHS.2006.320281

Fingerprint Dive into the research topics of 'Spatial and temporal application of microfluidics to cells'. Together they form a unique fingerprint.

Cite this

Yamada, A., Katanosaka, Y., Mohri, S., & Naruse, K. (2006). Spatial and temporal application of microfluidics to cells. In 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS [4110387] (2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS). https://doi.org/10.1109/MHS.2006.320281

Spatial and temporal application of microfluidics to cells. / Yamada, Akira; Katanosaka, Yuki; Mohri, Satoshi; Naruse, Keiji.

2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS. 2006. 4110387 (2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Yamada, A, Katanosaka, Y, Mohri, S & Naruse, K 2006, Spatial and temporal application of microfluidics to cells. in 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS., 4110387, 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS, 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS, Nagoya, Japan, 11/5/06. https://doi.org/10.1109/MHS.2006.320281

@inproceedings{3e8cb6a882d44edabcddbe11ce119342,

title = "Spatial and temporal application of microfluidics to cells",

abstract = "The rapid solution exchange around the adhesive single cell has recently received a great deal of attention from the viewpoint cell response analyses. We recently constructed a new system, which enables the replacement of solutions rapidly and precisely, by utilizing laminar flow technology. The sharp boundary of the adjoining two liquids (no mixing) moves around the cells, and minimum increments of the boundary shift are controlled at an interval of roughly 1.6 μm, and the time required for this movement is 20 msec. The system can be used for the analysis of intracellular Ca2+ concentrations using a fluorescent indicator, in which the cells are thus stimulated by the rapid exchange of the solution. These results suggest that the spatial and temporal control of the solution around the adhesive single cell can thus be realized.",

author = "Akira Yamada and Yuki Katanosaka and Satoshi Mohri and Keiji Naruse",

year = "2006",

month = dec,

day = "1",

doi = "10.1109/MHS.2006.320281",

language = "English",

isbn = "1424407176",

series = "2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS",

booktitle = "2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS",

note = "2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS ; Conference date: 05-11-2006 Through 08-11-2006",

}

TY - GEN

T1 - Spatial and temporal application of microfluidics to cells

AU - Yamada, Akira

AU - Katanosaka, Yuki

AU - Mohri, Satoshi

AU - Naruse, Keiji

PY - 2006/12/1

Y1 - 2006/12/1

N2 - The rapid solution exchange around the adhesive single cell has recently received a great deal of attention from the viewpoint cell response analyses. We recently constructed a new system, which enables the replacement of solutions rapidly and precisely, by utilizing laminar flow technology. The sharp boundary of the adjoining two liquids (no mixing) moves around the cells, and minimum increments of the boundary shift are controlled at an interval of roughly 1.6 μm, and the time required for this movement is 20 msec. The system can be used for the analysis of intracellular Ca2+ concentrations using a fluorescent indicator, in which the cells are thus stimulated by the rapid exchange of the solution. These results suggest that the spatial and temporal control of the solution around the adhesive single cell can thus be realized.

AB - The rapid solution exchange around the adhesive single cell has recently received a great deal of attention from the viewpoint cell response analyses. We recently constructed a new system, which enables the replacement of solutions rapidly and precisely, by utilizing laminar flow technology. The sharp boundary of the adjoining two liquids (no mixing) moves around the cells, and minimum increments of the boundary shift are controlled at an interval of roughly 1.6 μm, and the time required for this movement is 20 msec. The system can be used for the analysis of intracellular Ca2+ concentrations using a fluorescent indicator, in which the cells are thus stimulated by the rapid exchange of the solution. These results suggest that the spatial and temporal control of the solution around the adhesive single cell can thus be realized.

UR - http://www.scopus.com/inward/record.url?scp=50449093877&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50449093877&partnerID=8YFLogxK

U2 - 10.1109/MHS.2006.320281

DO - 10.1109/MHS.2006.320281

M3 - Conference contribution

AN - SCOPUS:50449093877

SN - 1424407176

SN - 9781424407170

T3 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS

BT - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS

T2 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS

Y2 - 5 November 2006 through 8 November 2006

ER -