Sparfloxacin resistance in clinical isolates of Streptococcus pneumoniae: Involvement of multiple mutations in gyrA and parC genes

Hideki Taba, Nobuchika Kusano

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38 Citations (Scopus)

Abstract

Antimicrobial susceptibility testing revealed among 150 clinical isolates of Streptococcus pneumoniae 4 pneumococcal isolates with resistance to fluoroquinolones (MIC of ciprofloxacin, ≤32 μg/ml; MIC of sparfloxacin, ≤16 μg/ml). Gene amplification and sequencing analysis of gyrA and parC revealed nucleotide changes leading to amine acid substitutions in both GyrA and Parc of all four fluoroquinolone-resistant isolates. In the case of strains 182 and 674 for which sparfloxacin MICs were 16 and 64 μg/ml, respectively, nucleotide changes were detected at codon 81 in gyrA and codon 79 in parC; these changes led to an Ser→Phe substitution in GyrA and an Ser→Phe substitution in ParC. Strains 354 and 252, for which sparfloxacin MICs were 128 μg/ml, revealed multiple mutations in both gyrA and parC. These strains exhibited nucleotide changes at codon 85 leading to a Glu→Lys substitution in GyrA, in addition to Ser-79→Tyr and Lys-137→Asn substitutions in ParC. Moreover, strain 252 showed additional nucleotide changes at codon 93, which led to a Trp→Arg substitution in GyrA. These results suggest that sparfloxacin resistance could be due to the multiple mutations in GyrA and ParC. However, it is possible that other yet unidentified mutations may also be involved in the high-level resistance to fluoroquinolones in S. pneumoniae.

Original languageEnglish
Pages (from-to)2193-2196
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume42
Issue number9
Publication statusPublished - Sep 1998
Externally publishedYes

Fingerprint

Streptococcus pneumoniae
Codon
Fluoroquinolones
Nucleotides
Mutation
Genes
Gene Amplification
Ciprofloxacin
Amines
Acids
sparfloxacin

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

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abstract = "Antimicrobial susceptibility testing revealed among 150 clinical isolates of Streptococcus pneumoniae 4 pneumococcal isolates with resistance to fluoroquinolones (MIC of ciprofloxacin, ≤32 μg/ml; MIC of sparfloxacin, ≤16 μg/ml). Gene amplification and sequencing analysis of gyrA and parC revealed nucleotide changes leading to amine acid substitutions in both GyrA and Parc of all four fluoroquinolone-resistant isolates. In the case of strains 182 and 674 for which sparfloxacin MICs were 16 and 64 μg/ml, respectively, nucleotide changes were detected at codon 81 in gyrA and codon 79 in parC; these changes led to an Ser→Phe substitution in GyrA and an Ser→Phe substitution in ParC. Strains 354 and 252, for which sparfloxacin MICs were 128 μg/ml, revealed multiple mutations in both gyrA and parC. These strains exhibited nucleotide changes at codon 85 leading to a Glu→Lys substitution in GyrA, in addition to Ser-79→Tyr and Lys-137→Asn substitutions in ParC. Moreover, strain 252 showed additional nucleotide changes at codon 93, which led to a Trp→Arg substitution in GyrA. These results suggest that sparfloxacin resistance could be due to the multiple mutations in GyrA and ParC. However, it is possible that other yet unidentified mutations may also be involved in the high-level resistance to fluoroquinolones in S. pneumoniae.",
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