TY - JOUR
T1 - Span 80 vesicles have a more fluid, flexible and " wet" surface than phospholipid liposomes
AU - Hayashi, Keita
AU - Shimanouchi, Toshinori
AU - Kato, Keiichi
AU - Miyazaki, Tatsuhiko
AU - Nakamura, Atsushi
AU - Umakoshi, Hiroshi
N1 - Funding Information:
The fundamental concept of this study was supported by the Research Group of “Membrane Stress Biotechnology” and the Sigma Multidisciplinary Research Laboratory (Graduate School of Engineering Science, Osaka University) “Membranomics.” The authors acknowledge Prof. Dr. Ryoichi Kuboi (Executive Director of San Francisco Center, Osaka University) for his helpful input on this research. The authors acknowledge Prof. Dr. Ryoichi Kuboi (Executive Director of San Francisco Center, Osaka University) and acknowledge Mr. Ryan Teel (Department of Chemical Engineering, University of South Carolina) for their experimental contributions. This research was partly supported by a Funding Program for Next Generation World-Leading Researchers of the Council for Science and Technology Policy (CSTP) (No. GR066 ), Grants-in-Aid for Scientific Research (Nos. 19656203 , 19656220 , and 20360350 ) from the Ministry of Education, Science, Sports, and Culture of Japan (MEXT) , and a grant from the Global COE program “Bio-Environmental Chemistry” of the Japan Society for the Promotion of Science (JSPS) . One of the authors (K.H.) acknowledges financial support as a GCOE fellow.
PY - 2011/10/1
Y1 - 2011/10/1
N2 - The surface properties of Span 80 vesicles at various cholesterol contents, together with those of various liposomes, were characterized by using fluorescence probes. The membrane fluidity of the Span 80 vesicles was measured by 1,6-diphenyl-1.3.5-hexatriene (DPH) and trimethlyammonium-DPH (TMA-DPH), and the results suggested that the surface of the Span 80 vesicles was fluid due to the lateral diffusion of Span 80 molecules. The depolarization measured by TMA-DPH and the headgroup mobility measured by dielectric dispersion analysis indicated the high mobility of the head group of Span 80 vesicles. This suggested that the surface of Span 80 vesicles was flexible due to the head group structure of Span 80, sorbitol. In addition, spectrophotometric analysis with 6-dodecanoyl-N, N-dimethyl-2-naphthylamine and 8-anilino-1-naphthalenesulfonic acid indicated that the water molecules could easily invade into the interior of the Span 80 vesicle membrane, suggesting that the membrane surface was more wet than the liposome surface. These surface properties indicated that the protein could interact with the interior of vesicle membranes, which was similar to the case of cholesterol. Thus the present results confirmed that the Span 80 vesicle surfaces showed the unique characteristics of fluidity, flexibility, and " wetness" , whereas the liposome surfaces did not.
AB - The surface properties of Span 80 vesicles at various cholesterol contents, together with those of various liposomes, were characterized by using fluorescence probes. The membrane fluidity of the Span 80 vesicles was measured by 1,6-diphenyl-1.3.5-hexatriene (DPH) and trimethlyammonium-DPH (TMA-DPH), and the results suggested that the surface of the Span 80 vesicles was fluid due to the lateral diffusion of Span 80 molecules. The depolarization measured by TMA-DPH and the headgroup mobility measured by dielectric dispersion analysis indicated the high mobility of the head group of Span 80 vesicles. This suggested that the surface of Span 80 vesicles was flexible due to the head group structure of Span 80, sorbitol. In addition, spectrophotometric analysis with 6-dodecanoyl-N, N-dimethyl-2-naphthylamine and 8-anilino-1-naphthalenesulfonic acid indicated that the water molecules could easily invade into the interior of the Span 80 vesicle membrane, suggesting that the membrane surface was more wet than the liposome surface. These surface properties indicated that the protein could interact with the interior of vesicle membranes, which was similar to the case of cholesterol. Thus the present results confirmed that the Span 80 vesicle surfaces showed the unique characteristics of fluidity, flexibility, and " wetness" , whereas the liposome surfaces did not.
KW - Membranome
KW - Vesicle
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U2 - 10.1016/j.colsurfb.2011.04.029
DO - 10.1016/j.colsurfb.2011.04.029
M3 - Article
C2 - 21621983
AN - SCOPUS:79959349765
VL - 87
SP - 28
EP - 35
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 1
ER -