Span 80 vesicles have a more fluid, flexible and " wet" surface than phospholipid liposomes

Keita Hayashi, Toshinori Shimanouchi, Keiichi Kato, Tatsuhiko Miyazaki, Atsushi Nakamura, Hiroshi Umakoshi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The surface properties of Span 80 vesicles at various cholesterol contents, together with those of various liposomes, were characterized by using fluorescence probes. The membrane fluidity of the Span 80 vesicles was measured by 1,6-diphenyl-1.3.5-hexatriene (DPH) and trimethlyammonium-DPH (TMA-DPH), and the results suggested that the surface of the Span 80 vesicles was fluid due to the lateral diffusion of Span 80 molecules. The depolarization measured by TMA-DPH and the headgroup mobility measured by dielectric dispersion analysis indicated the high mobility of the head group of Span 80 vesicles. This suggested that the surface of Span 80 vesicles was flexible due to the head group structure of Span 80, sorbitol. In addition, spectrophotometric analysis with 6-dodecanoyl-N, N-dimethyl-2-naphthylamine and 8-anilino-1-naphthalenesulfonic acid indicated that the water molecules could easily invade into the interior of the Span 80 vesicle membrane, suggesting that the membrane surface was more wet than the liposome surface. These surface properties indicated that the protein could interact with the interior of vesicle membranes, which was similar to the case of cholesterol. Thus the present results confirmed that the Span 80 vesicle surfaces showed the unique characteristics of fluidity, flexibility, and " wetness" , whereas the liposome surfaces did not.

Original languageEnglish
Pages (from-to)28-35
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume87
Issue number1
DOIs
Publication statusPublished - Oct 1 2011
Externally publishedYes

Fingerprint

Liposomes
Phospholipids
Fluids
fluids
Membranes
Fluidity
Cholesterol
membranes
Surface properties
Surface Properties
cholesterol
surface properties
Molecules
Depolarization
2-Naphthylamine
sorbitan monooleate
Membrane Fluidity
Permittivity
Sorbitol
Fluorescence

Keywords

  • Membranome
  • Vesicle

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Span 80 vesicles have a more fluid, flexible and " wet" surface than phospholipid liposomes. / Hayashi, Keita; Shimanouchi, Toshinori; Kato, Keiichi; Miyazaki, Tatsuhiko; Nakamura, Atsushi; Umakoshi, Hiroshi.

In: Colloids and Surfaces B: Biointerfaces, Vol. 87, No. 1, 01.10.2011, p. 28-35.

Research output: Contribution to journalArticle

Hayashi, Keita ; Shimanouchi, Toshinori ; Kato, Keiichi ; Miyazaki, Tatsuhiko ; Nakamura, Atsushi ; Umakoshi, Hiroshi. / Span 80 vesicles have a more fluid, flexible and " wet" surface than phospholipid liposomes. In: Colloids and Surfaces B: Biointerfaces. 2011 ; Vol. 87, No. 1. pp. 28-35.
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