Abstract
Veins are longitudinal cuticular structures that maintain shape of the wing. . Drosophila melanogaster has six longitudinal veins (L1-L6) and two cross veins. The Zn-finger transcription factors of Spalt-complex (Sal) are required for positioning of the L2 and L5, and the homeodomain transcription factors of Iroquois complex (Iro-C) are required for formation of the L3 and L5 veins.The homeodomain transcriptional repressor Defective proventriculus (Dve) is uniformly expressed in the wing pouch of the larval imaginal disc. However, . dve mutant wings showed loss of the L2 and L5, but not of the L3 and L4 veins. Temporal . dve knockdown experiments indicate that the Dve activity is required for vein formation from late third larval instar to the prepupal stage. In the prepupal wing, Dve expression becomes nearly complementary to that of Sal through the Sal-mediated . dve repression. Furthermore, coexpression of Dve and Iro-C relieved of Sal-mediated repression is required for the L5 formation in a dose-dependent manner. The relationship between Sal, Dve, and Iro-C in wing vein specification is quite similar to that in ommatidial cell-type specification. Our results provide information about the conserved function of . dve regulatory motifs in cell differentiation.
| Original language | English |
|---|---|
| Journal | Mechanisms of Development |
| DOIs | |
| Publication status | Accepted/In press - Jan 13 2016 |
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Keywords
- Dpp
- Drosophila
- Iroquois
- Spalt
- Transcription factor
- Wing vein
ASJC Scopus subject areas
- Developmental Biology
- Embryology
Cite this
Spalt-mediated dve repression is a critical regulatory motif and coordinates with Iroquois complex in Drosophila vein formation. / Sugimori, Seiko; Hasegawa, Aya; Nakagoshi, Hideki.
In: Mechanisms of Development, 13.01.2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Spalt-mediated dve repression is a critical regulatory motif and coordinates with Iroquois complex in Drosophila vein formation
AU - Sugimori, Seiko
AU - Hasegawa, Aya
AU - Nakagoshi, Hideki
PY - 2016/1/13
Y1 - 2016/1/13
N2 - Veins are longitudinal cuticular structures that maintain shape of the wing. . Drosophila melanogaster has six longitudinal veins (L1-L6) and two cross veins. The Zn-finger transcription factors of Spalt-complex (Sal) are required for positioning of the L2 and L5, and the homeodomain transcription factors of Iroquois complex (Iro-C) are required for formation of the L3 and L5 veins.The homeodomain transcriptional repressor Defective proventriculus (Dve) is uniformly expressed in the wing pouch of the larval imaginal disc. However, . dve mutant wings showed loss of the L2 and L5, but not of the L3 and L4 veins. Temporal . dve knockdown experiments indicate that the Dve activity is required for vein formation from late third larval instar to the prepupal stage. In the prepupal wing, Dve expression becomes nearly complementary to that of Sal through the Sal-mediated . dve repression. Furthermore, coexpression of Dve and Iro-C relieved of Sal-mediated repression is required for the L5 formation in a dose-dependent manner. The relationship between Sal, Dve, and Iro-C in wing vein specification is quite similar to that in ommatidial cell-type specification. Our results provide information about the conserved function of . dve regulatory motifs in cell differentiation.
AB - Veins are longitudinal cuticular structures that maintain shape of the wing. . Drosophila melanogaster has six longitudinal veins (L1-L6) and two cross veins. The Zn-finger transcription factors of Spalt-complex (Sal) are required for positioning of the L2 and L5, and the homeodomain transcription factors of Iroquois complex (Iro-C) are required for formation of the L3 and L5 veins.The homeodomain transcriptional repressor Defective proventriculus (Dve) is uniformly expressed in the wing pouch of the larval imaginal disc. However, . dve mutant wings showed loss of the L2 and L5, but not of the L3 and L4 veins. Temporal . dve knockdown experiments indicate that the Dve activity is required for vein formation from late third larval instar to the prepupal stage. In the prepupal wing, Dve expression becomes nearly complementary to that of Sal through the Sal-mediated . dve repression. Furthermore, coexpression of Dve and Iro-C relieved of Sal-mediated repression is required for the L5 formation in a dose-dependent manner. The relationship between Sal, Dve, and Iro-C in wing vein specification is quite similar to that in ommatidial cell-type specification. Our results provide information about the conserved function of . dve regulatory motifs in cell differentiation.
KW - Dpp
KW - Drosophila
KW - Iroquois
KW - Spalt
KW - Transcription factor
KW - Wing vein
UR - http://www.scopus.com/inward/record.url?scp=84978531789&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84978531789&partnerID=8YFLogxK
U2 - 10.1016/j.mod.2016.06.004
DO - 10.1016/j.mod.2016.06.004
M3 - Article
C2 - 27349585
AN - SCOPUS:84978531789
JO - Mechanisms of Development
JF - Mechanisms of Development
SN - 0925-4773
ER -