TY - JOUR
T1 - Spalt-mediated dve repression is a critical regulatory motif and coordinates with Iroquois complex in Drosophila vein formation
AU - Sugimori, Seiko
AU - Hasegawa, Aya
AU - Nakagoshi, Hideki
N1 - Funding Information:
We are grateful to the Bloomington Stock Center, the Drosophila Genetic Resource Center (DGRC, Kyoto), and the Vienna Drosophila Resource Center (VDRC) for fly strains. We also thank the Advanced Science Research Center (Okayama University) for the use of confocal microscope OLYMPUS FV300 and fluorescence microscope KEYENCE BZ-9000. This work was partially supported by JSPS KAKENHI Grant Number 16027233 (to H. N.).
Publisher Copyright:
© 2016 Elsevier Ireland Ltd
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Veins are longitudinal cuticular structures that maintain shape of the wing. Drosophila melanogaster has six longitudinal veins (L1–L6) and two cross veins. The Zn-finger transcription factors of Spalt-complex (Sal) are required for positioning of the L2 and L5, and the homeodomain transcription factors of Iroquois complex (Iro-C) are required for formation of the L3 and L5 veins. The homeodomain transcriptional repressor Defective proventriculus (Dve) is uniformly expressed in the wing pouch of the larval imaginal disc. However, dve mutant wings showed loss of the L2 and L5, but not of the L3 and L4 veins. Temporal dve knockdown experiments indicate that the Dve activity is required for vein formation from late third larval instar to the prepupal stage. In the prepupal wing, Dve expression becomes nearly complementary to that of Sal through the Sal-mediated dve repression. Furthermore, coexpression of Dve and Iro-C relieved of Sal-mediated repression is required for the L5 formation in a dose-dependent manner. The relationship between Sal, Dve, and Iro-C in wing vein specification is quite similar to that in ommatidial cell-type specification. Our results provide information about the conserved function of dve regulatory motifs in cell differentiation.
AB - Veins are longitudinal cuticular structures that maintain shape of the wing. Drosophila melanogaster has six longitudinal veins (L1–L6) and two cross veins. The Zn-finger transcription factors of Spalt-complex (Sal) are required for positioning of the L2 and L5, and the homeodomain transcription factors of Iroquois complex (Iro-C) are required for formation of the L3 and L5 veins. The homeodomain transcriptional repressor Defective proventriculus (Dve) is uniformly expressed in the wing pouch of the larval imaginal disc. However, dve mutant wings showed loss of the L2 and L5, but not of the L3 and L4 veins. Temporal dve knockdown experiments indicate that the Dve activity is required for vein formation from late third larval instar to the prepupal stage. In the prepupal wing, Dve expression becomes nearly complementary to that of Sal through the Sal-mediated dve repression. Furthermore, coexpression of Dve and Iro-C relieved of Sal-mediated repression is required for the L5 formation in a dose-dependent manner. The relationship between Sal, Dve, and Iro-C in wing vein specification is quite similar to that in ommatidial cell-type specification. Our results provide information about the conserved function of dve regulatory motifs in cell differentiation.
KW - Dpp
KW - Drosophila
KW - Iroquois
KW - Spalt
KW - Transcription factor
KW - Wing vein
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U2 - 10.1016/j.mod.2016.06.004
DO - 10.1016/j.mod.2016.06.004
M3 - Article
C2 - 27349585
AN - SCOPUS:84978531789
VL - 141
SP - 25
EP - 31
JO - Cells and Development
JF - Cells and Development
SN - 2667-291X
ER -