TY - JOUR
T1 - Soy and Arabidopsis receptor-like kinases respond to polysaccharide signals from Spodoptera species and mediate herbivore resistance
AU - Uemura, Takuya
AU - Hachisu, Masakazu
AU - Desaki, Yoshitake
AU - Ito, Ayaka
AU - Hoshino, Ryosuke
AU - Sano, Yuka
AU - Nozawa, Akira
AU - Mujiono, Kadis
AU - Galis, Ivan
AU - Yoshida, Ayako
AU - Nemoto, Keiichirou
AU - Miura, Shigetoshi
AU - Nishiyama, Makoto
AU - Nishiyama, Chiharu
AU - Horito, Shigeomi
AU - Sawasaki, Tatsuya
AU - Arimura, Gen ichiro
N1 - Funding Information:
We would like to thank Dr. Hanae Kaku (Meiji University) for kindly providing cerk1 seeds and chitin oligosaccharides [(GlcNAc)7]. 18:3-Gln, M. loreyi OS, atpbl27 seeds were provided by Dr. Tomonori Shinya (Okayama University). The seeds of bik1/pbl1 were provided by Dr. Jian-Min Zhou (Chinese Academy of Sciences). The seeds of xlg1/2/3 were provided by Dr. Shinichiro Sawa (Kumamoto University). The vectors pGWB405, pGWB451, and pGWB452 were provided by Dr. Tsuyoshi Nakagawa (Shimane University). The virus vectors (pEALSR1 and pEALSR2L5R5) and BiFC vectors were provided by Dr. Nobuyuki Yoshikawa (Iwate University) and Dr. Jörg Kudla (University of Münster), respectively. Phytohormone analyses were assisted by Ms. Yuko Hojo (Okayama University). This work was financially supported in part by MEXT Grants-in-Aid for Scientific Research on Innovative Areas to G.A. (18H04786 and 20H04786), JSPS KAKENHI to T.U. (20K15878), Y.D. (20K06058), and G.A. (20H02951), and by the Joint Usage/Research Center, Institute of Plant Science and Resources, Okayama University.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Plants respond to herbivory by perceiving herbivore danger signal(s) (HDS(s)), including “elicitors”, that are present in herbivores’ oral secretions (OS) and act to induce defense responses. However, little is known about HDS-specific molecules and intracellular signaling. Here we explored soybean receptor-like kinases (RLKs) as candidates that might mediate HDS-associated RLKs’ (HAKs’) actions in leaves in response to OS extracted from larvae of a generalist herbivore, Spodoptera litura. Fractionation of OS yielded Frα, which consisted of polysaccharides. The GmHAKs composed of their respective homomultimers scarcely interacted with Frα. Moreover, Arabidopsis HAK1 homomultimers interacted with cytoplasmic signaling molecule PBL27, resulting in herbivory resistance, in an ethylene-dependent manner. Altogether, our findings suggest that HAKs are herbivore-specific RLKs mediating HDS-transmitting, intracellular signaling through interaction with PBL27 and the subsequent ethylene signaling for plant defense responses in host plants.
AB - Plants respond to herbivory by perceiving herbivore danger signal(s) (HDS(s)), including “elicitors”, that are present in herbivores’ oral secretions (OS) and act to induce defense responses. However, little is known about HDS-specific molecules and intracellular signaling. Here we explored soybean receptor-like kinases (RLKs) as candidates that might mediate HDS-associated RLKs’ (HAKs’) actions in leaves in response to OS extracted from larvae of a generalist herbivore, Spodoptera litura. Fractionation of OS yielded Frα, which consisted of polysaccharides. The GmHAKs composed of their respective homomultimers scarcely interacted with Frα. Moreover, Arabidopsis HAK1 homomultimers interacted with cytoplasmic signaling molecule PBL27, resulting in herbivory resistance, in an ethylene-dependent manner. Altogether, our findings suggest that HAKs are herbivore-specific RLKs mediating HDS-transmitting, intracellular signaling through interaction with PBL27 and the subsequent ethylene signaling for plant defense responses in host plants.
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U2 - 10.1038/s42003-020-0959-4
DO - 10.1038/s42003-020-0959-4
M3 - Article
C2 - 32385340
AN - SCOPUS:85084324387
VL - 3
JO - Communications Biology
JF - Communications Biology
SN - 2399-3642
IS - 1
M1 - 224
ER -