Sorcin expression in the epiphyseal growth plates of mice

Mariko Kawai, Ning Liu, Takako Hattori, Yo Hei Kataoka, Masaharu Takigawa, Satoshi Kubota, Toshio Yamamoto, Kiyoshi Ohura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Sorcin is a small calcium-binding protein that is widely expressed in many tissues. Sorcin regulates cardiac myocyte apoptosis by modulating mitochondrial Ca2+ cycling and regulates fibroblast cell cycling and apoptosis via Ca2+ signaling through the endoplasmic reticulum (ER). During endochondral ossification, some chondrocytes undergo apoptosis after their terminal differentiation; however, Sorcin’s expression in these cells has not been characterized. Here we examined Sorcin’s gene expression in the chondrocytes derived from mouse growth plate by reverse transcription PCR (RT-PCR), and its protein localization in the chondrocytes of femoral growth plate using immunohistochemistry. Sorcin protein was detected in the chondrocytes, and was particularly abundant in the cytoplasm and nuclei of proliferative zone chondrocytes and in the nuclei of hypertrophic chondrocytes. Apoptotic analysis of the growth plate revealed that many of the hypertrophic chondrocytes undergo the DNA fragmentation. We report for the first time the localization of Sorcin in the epiphyseal growth plate in which one of the apoptotic phenomenon was detected.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalJournal of Hard Tissue Biology
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 15 2016

Fingerprint

Growth Plate
Chondrocytes
Cell death
Apoptosis
Proteins
Calcium-Binding Proteins
Transcription
Fibroblasts
Gene expression
Calcium
DNA
Cells
Tissue
DNA Fragmentation
Thigh
Cardiac Myocytes
Osteogenesis
Endoplasmic Reticulum
Reverse Transcription
Cytoplasm

Keywords

  • Apoptosis
  • Chondrocyte
  • Epiphyseal growth plate
  • Sorcin

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

Sorcin expression in the epiphyseal growth plates of mice. / Kawai, Mariko; Liu, Ning; Hattori, Takako; Kataoka, Yo Hei; Takigawa, Masaharu; Kubota, Satoshi; Yamamoto, Toshio; Ohura, Kiyoshi.

In: Journal of Hard Tissue Biology, Vol. 25, No. 1, 15.01.2016, p. 57-62.

Research output: Contribution to journalArticle

Kawai, Mariko ; Liu, Ning ; Hattori, Takako ; Kataoka, Yo Hei ; Takigawa, Masaharu ; Kubota, Satoshi ; Yamamoto, Toshio ; Ohura, Kiyoshi. / Sorcin expression in the epiphyseal growth plates of mice. In: Journal of Hard Tissue Biology. 2016 ; Vol. 25, No. 1. pp. 57-62.
@article{44223cb455db4c35b35a1bfdea5fbf20,
title = "Sorcin expression in the epiphyseal growth plates of mice",
abstract = "Sorcin is a small calcium-binding protein that is widely expressed in many tissues. Sorcin regulates cardiac myocyte apoptosis by modulating mitochondrial Ca2+ cycling and regulates fibroblast cell cycling and apoptosis via Ca2+ signaling through the endoplasmic reticulum (ER). During endochondral ossification, some chondrocytes undergo apoptosis after their terminal differentiation; however, Sorcin’s expression in these cells has not been characterized. Here we examined Sorcin’s gene expression in the chondrocytes derived from mouse growth plate by reverse transcription PCR (RT-PCR), and its protein localization in the chondrocytes of femoral growth plate using immunohistochemistry. Sorcin protein was detected in the chondrocytes, and was particularly abundant in the cytoplasm and nuclei of proliferative zone chondrocytes and in the nuclei of hypertrophic chondrocytes. Apoptotic analysis of the growth plate revealed that many of the hypertrophic chondrocytes undergo the DNA fragmentation. We report for the first time the localization of Sorcin in the epiphyseal growth plate in which one of the apoptotic phenomenon was detected.",
keywords = "Apoptosis, Chondrocyte, Epiphyseal growth plate, Sorcin",
author = "Mariko Kawai and Ning Liu and Takako Hattori and Kataoka, {Yo Hei} and Masaharu Takigawa and Satoshi Kubota and Toshio Yamamoto and Kiyoshi Ohura",
year = "2016",
month = "1",
day = "15",
doi = "10.2485/jhtb.25.57",
language = "English",
volume = "25",
pages = "57--62",
journal = "Journal of Hard Tissue Biology",
issn = "1341-7649",
publisher = "Society of Hard Tissue Regenerative Biology",
number = "1",

}

TY - JOUR

T1 - Sorcin expression in the epiphyseal growth plates of mice

AU - Kawai, Mariko

AU - Liu, Ning

AU - Hattori, Takako

AU - Kataoka, Yo Hei

AU - Takigawa, Masaharu

AU - Kubota, Satoshi

AU - Yamamoto, Toshio

AU - Ohura, Kiyoshi

PY - 2016/1/15

Y1 - 2016/1/15

N2 - Sorcin is a small calcium-binding protein that is widely expressed in many tissues. Sorcin regulates cardiac myocyte apoptosis by modulating mitochondrial Ca2+ cycling and regulates fibroblast cell cycling and apoptosis via Ca2+ signaling through the endoplasmic reticulum (ER). During endochondral ossification, some chondrocytes undergo apoptosis after their terminal differentiation; however, Sorcin’s expression in these cells has not been characterized. Here we examined Sorcin’s gene expression in the chondrocytes derived from mouse growth plate by reverse transcription PCR (RT-PCR), and its protein localization in the chondrocytes of femoral growth plate using immunohistochemistry. Sorcin protein was detected in the chondrocytes, and was particularly abundant in the cytoplasm and nuclei of proliferative zone chondrocytes and in the nuclei of hypertrophic chondrocytes. Apoptotic analysis of the growth plate revealed that many of the hypertrophic chondrocytes undergo the DNA fragmentation. We report for the first time the localization of Sorcin in the epiphyseal growth plate in which one of the apoptotic phenomenon was detected.

AB - Sorcin is a small calcium-binding protein that is widely expressed in many tissues. Sorcin regulates cardiac myocyte apoptosis by modulating mitochondrial Ca2+ cycling and regulates fibroblast cell cycling and apoptosis via Ca2+ signaling through the endoplasmic reticulum (ER). During endochondral ossification, some chondrocytes undergo apoptosis after their terminal differentiation; however, Sorcin’s expression in these cells has not been characterized. Here we examined Sorcin’s gene expression in the chondrocytes derived from mouse growth plate by reverse transcription PCR (RT-PCR), and its protein localization in the chondrocytes of femoral growth plate using immunohistochemistry. Sorcin protein was detected in the chondrocytes, and was particularly abundant in the cytoplasm and nuclei of proliferative zone chondrocytes and in the nuclei of hypertrophic chondrocytes. Apoptotic analysis of the growth plate revealed that many of the hypertrophic chondrocytes undergo the DNA fragmentation. We report for the first time the localization of Sorcin in the epiphyseal growth plate in which one of the apoptotic phenomenon was detected.

KW - Apoptosis

KW - Chondrocyte

KW - Epiphyseal growth plate

KW - Sorcin

UR - http://www.scopus.com/inward/record.url?scp=84954432966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84954432966&partnerID=8YFLogxK

U2 - 10.2485/jhtb.25.57

DO - 10.2485/jhtb.25.57

M3 - Article

AN - SCOPUS:84954432966

VL - 25

SP - 57

EP - 62

JO - Journal of Hard Tissue Biology

JF - Journal of Hard Tissue Biology

SN - 1341-7649

IS - 1

ER -