Some properties of purified Escherichia coli heat-stable enterotoxin II

S. Hitotsubashi, Y. Fujii, H. Yamanaka, Keinosuke Okamoto

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

We examined the biological properties of purified Escherichia coli heat- stable enterotoxin II (STII) using mouse intestinal loop assays and compared these properties with those of heat-stable enterotoxin I (STI) and cholera toxin (CT). The action of STII over time differed from those of STI and CT. STII did not alter cyclic GMP or cyclic AMP levels in intestinal mucosal cells. Our results supported the idea that the mechanism of action of STII in inducing fluid secretion is different from the mechanisms of action of STI and CT. This hypothesis was further supported by the fact that an anti-STII neutralizing serum did not neutralize the activities of STI and CT. Subsequently, we examined the involvement of prostaglandins in the action of STII. The level of prostaglandin E2 in the fluid accumulated as a result of the action of STII increased, and the prostaglandin synthesis inhibitors aspirin and indomethacin significantly reduced the response to STII. These results implicate prostaglandin E2 in the mechanism of action of STII.

Original languageEnglish
Pages (from-to)4468-4474
Number of pages7
JournalInfection and Immunity
Volume60
Issue number11
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

Enterotoxins
Escherichia coli
Cholera Toxin
Dinoprostone
heat stable toxin (E coli)
Fluids and Secretions
Prostaglandin Antagonists
Cyclic GMP
Indomethacin
Cyclic AMP
Aspirin
Prostaglandins

ASJC Scopus subject areas

  • Immunology

Cite this

Hitotsubashi, S., Fujii, Y., Yamanaka, H., & Okamoto, K. (1992). Some properties of purified Escherichia coli heat-stable enterotoxin II. Infection and Immunity, 60(11), 4468-4474.

Some properties of purified Escherichia coli heat-stable enterotoxin II. / Hitotsubashi, S.; Fujii, Y.; Yamanaka, H.; Okamoto, Keinosuke.

In: Infection and Immunity, Vol. 60, No. 11, 1992, p. 4468-4474.

Research output: Contribution to journalArticle

Hitotsubashi, S, Fujii, Y, Yamanaka, H & Okamoto, K 1992, 'Some properties of purified Escherichia coli heat-stable enterotoxin II', Infection and Immunity, vol. 60, no. 11, pp. 4468-4474.
Hitotsubashi, S. ; Fujii, Y. ; Yamanaka, H. ; Okamoto, Keinosuke. / Some properties of purified Escherichia coli heat-stable enterotoxin II. In: Infection and Immunity. 1992 ; Vol. 60, No. 11. pp. 4468-4474.
@article{f248539cbcf04cca89829885263c105d,
title = "Some properties of purified Escherichia coli heat-stable enterotoxin II",
abstract = "We examined the biological properties of purified Escherichia coli heat- stable enterotoxin II (STII) using mouse intestinal loop assays and compared these properties with those of heat-stable enterotoxin I (STI) and cholera toxin (CT). The action of STII over time differed from those of STI and CT. STII did not alter cyclic GMP or cyclic AMP levels in intestinal mucosal cells. Our results supported the idea that the mechanism of action of STII in inducing fluid secretion is different from the mechanisms of action of STI and CT. This hypothesis was further supported by the fact that an anti-STII neutralizing serum did not neutralize the activities of STI and CT. Subsequently, we examined the involvement of prostaglandins in the action of STII. The level of prostaglandin E2 in the fluid accumulated as a result of the action of STII increased, and the prostaglandin synthesis inhibitors aspirin and indomethacin significantly reduced the response to STII. These results implicate prostaglandin E2 in the mechanism of action of STII.",
author = "S. Hitotsubashi and Y. Fujii and H. Yamanaka and Keinosuke Okamoto",
year = "1992",
language = "English",
volume = "60",
pages = "4468--4474",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "11",

}

TY - JOUR

T1 - Some properties of purified Escherichia coli heat-stable enterotoxin II

AU - Hitotsubashi, S.

AU - Fujii, Y.

AU - Yamanaka, H.

AU - Okamoto, Keinosuke

PY - 1992

Y1 - 1992

N2 - We examined the biological properties of purified Escherichia coli heat- stable enterotoxin II (STII) using mouse intestinal loop assays and compared these properties with those of heat-stable enterotoxin I (STI) and cholera toxin (CT). The action of STII over time differed from those of STI and CT. STII did not alter cyclic GMP or cyclic AMP levels in intestinal mucosal cells. Our results supported the idea that the mechanism of action of STII in inducing fluid secretion is different from the mechanisms of action of STI and CT. This hypothesis was further supported by the fact that an anti-STII neutralizing serum did not neutralize the activities of STI and CT. Subsequently, we examined the involvement of prostaglandins in the action of STII. The level of prostaglandin E2 in the fluid accumulated as a result of the action of STII increased, and the prostaglandin synthesis inhibitors aspirin and indomethacin significantly reduced the response to STII. These results implicate prostaglandin E2 in the mechanism of action of STII.

AB - We examined the biological properties of purified Escherichia coli heat- stable enterotoxin II (STII) using mouse intestinal loop assays and compared these properties with those of heat-stable enterotoxin I (STI) and cholera toxin (CT). The action of STII over time differed from those of STI and CT. STII did not alter cyclic GMP or cyclic AMP levels in intestinal mucosal cells. Our results supported the idea that the mechanism of action of STII in inducing fluid secretion is different from the mechanisms of action of STI and CT. This hypothesis was further supported by the fact that an anti-STII neutralizing serum did not neutralize the activities of STI and CT. Subsequently, we examined the involvement of prostaglandins in the action of STII. The level of prostaglandin E2 in the fluid accumulated as a result of the action of STII increased, and the prostaglandin synthesis inhibitors aspirin and indomethacin significantly reduced the response to STII. These results implicate prostaglandin E2 in the mechanism of action of STII.

UR - http://www.scopus.com/inward/record.url?scp=0026484874&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026484874&partnerID=8YFLogxK

M3 - Article

VL - 60

SP - 4468

EP - 4474

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 11

ER -