Some properties of a novel obligately autotrophic iron-oxidizing bacterium isolated from seawater

Kazuo Kamimura, K. Kunomura, S. Wakai, K. Murakami, T. Sugio

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An iron-oxidizing bacterium obligately requiring NaCl for growth was isolated from seawater in Seto Inland Sea, Japan, and designated as strain KU2-11. Strain KU2-11 was a Gram negative, non-spore-forming and rod-shaped bacterium, and can grow autotrophically by using ferrous ion and elemental sulfur as sole energy sources. The optimum growth pH and temperature were 2 and 30 °C, respectively. The G+C content of the DNA was 59 mol%. The bacterium could not grow in the medium without NaCl and obligately required NaCl for growth. The optimum NaCl concentration for the growth was 2%. Strain KU2-11 seemed to be identified as a member of Thiobacillus ferrooxidans on the basis of morphological and physiological properties. However, the phylogenetic analysis based on 16S rDNA gene sequences indicated that strain KU2-11 is distinct from T. ferrooxidans. Iron- and sulfur-oxidizing activity of the bacterium were not detected in a reaction mixture without NaCl, and depended on the presence of NaCl. The optimum NaCl concentrations for the iron- and sulfur-oxidizing activities were 2-4% and 1%, respectively. On the basis of the phenotypic characteristics of strain KU2-11 and its phylogenetic position, we suggested that this bacterium should be placed in a new species of the genus Thiobacillus.

Original languageEnglish
Pages (from-to)373-381
Number of pages9
JournalHydrometallurgy
Volume59
Issue number2-3
DOIs
Publication statusPublished - Feb 2001

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Seawater
Bacteria
Iron
seawater
iron
bacterium
Sulfur
sulfur
phylogenetics
inland sea
Ribosomal DNA
DNA
Genes
Ions
new species
ion
gene
temperature
Temperature

ASJC Scopus subject areas

  • Geochemistry and Petrology
  • Geotechnical Engineering and Engineering Geology

Cite this

Some properties of a novel obligately autotrophic iron-oxidizing bacterium isolated from seawater. / Kamimura, Kazuo; Kunomura, K.; Wakai, S.; Murakami, K.; Sugio, T.

In: Hydrometallurgy, Vol. 59, No. 2-3, 02.2001, p. 373-381.

Research output: Contribution to journalArticle

Kamimura, Kazuo ; Kunomura, K. ; Wakai, S. ; Murakami, K. ; Sugio, T. / Some properties of a novel obligately autotrophic iron-oxidizing bacterium isolated from seawater. In: Hydrometallurgy. 2001 ; Vol. 59, No. 2-3. pp. 373-381.
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