Abstract
The objective of this study was to detect soluble-form tumour necrosis factor receptors (sTNFRs) in temporomandibular joint (TMJ) synovial fluid aspirates, and to compare the sTNFR concentrations between painful anterior disc displacement without reduction and osteoarthritis (ADDwoR/OA) and asymptomatic TMJs. Synovial fluid was sampled from the superior TMJ cavity of 11 painful ADDwoR/OA cases (mean age: 36.9 years) and 10 asymptomatic females (mean age: 24.7 years) by diluted aspiration. The concentrations of sTNFR-I and -II in the synovial fluid were measured using human sTNFR-I and -II enzyme-linked immunosorbent assays. The total protein concentrations in synovial fluids were measured using a bicinchoninic acid protein assay kit. All data were normalised to the total protein concentration of each sample. Two-way factorial analysis of variance and post hoc multiple comparison revealed that: (1) mean normalised sTNFR-I and -II concentrations were higher in TMJ synovial aspirates from ADDwoR/OA patients than from healthy controls; (2) in the ADDwoR/OA patients and the healthy controls, the sTNFR-I concentration in TMJ synovial aspirates was higher than the sTNFR-II concentration; and (3) high TMJ synovial aspirate sTNFR-II seemed to be associated with less TMJ pain and a less restricted range of mouth opening in the ADDwoR/OA patients. The concentrations of sTNFRs in TMJ synovial fluid are higher in the presence of painful ADDwoR/OA, which could modulate intracapsular inflammation.
| Original language | English |
|---|---|
| Pages (from-to) | 133-142 |
| Number of pages | 10 |
| Journal | Archives of Oral Biology |
| Volume | 49 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2004 |
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Keywords
- Osteoarthritis (OA)
- Soluble-form receptor
- Synovial fluid
- Temporomandibular joint (TMJ)
- TNFα
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Soluble tumour necrosis factor receptors in synovial fluids from temporomandibular joints with painful anterior disc displacement without reduction and osteoarthritis. / Uehara, Junji; Kuboki, Takuo; Fujisawa, Takuo; Kojima, Shunji; Maekawa, Kenji; Yatani, Hirofumi.
In: Archives of Oral Biology, Vol. 49, No. 2, 02.2004, p. 133-142.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Soluble tumour necrosis factor receptors in synovial fluids from temporomandibular joints with painful anterior disc displacement without reduction and osteoarthritis
AU - Uehara, Junji
AU - Kuboki, Takuo
AU - Fujisawa, Takuo
AU - Kojima, Shunji
AU - Maekawa, Kenji
AU - Yatani, Hirofumi
PY - 2004/2
Y1 - 2004/2
N2 - The objective of this study was to detect soluble-form tumour necrosis factor receptors (sTNFRs) in temporomandibular joint (TMJ) synovial fluid aspirates, and to compare the sTNFR concentrations between painful anterior disc displacement without reduction and osteoarthritis (ADDwoR/OA) and asymptomatic TMJs. Synovial fluid was sampled from the superior TMJ cavity of 11 painful ADDwoR/OA cases (mean age: 36.9 years) and 10 asymptomatic females (mean age: 24.7 years) by diluted aspiration. The concentrations of sTNFR-I and -II in the synovial fluid were measured using human sTNFR-I and -II enzyme-linked immunosorbent assays. The total protein concentrations in synovial fluids were measured using a bicinchoninic acid protein assay kit. All data were normalised to the total protein concentration of each sample. Two-way factorial analysis of variance and post hoc multiple comparison revealed that: (1) mean normalised sTNFR-I and -II concentrations were higher in TMJ synovial aspirates from ADDwoR/OA patients than from healthy controls; (2) in the ADDwoR/OA patients and the healthy controls, the sTNFR-I concentration in TMJ synovial aspirates was higher than the sTNFR-II concentration; and (3) high TMJ synovial aspirate sTNFR-II seemed to be associated with less TMJ pain and a less restricted range of mouth opening in the ADDwoR/OA patients. The concentrations of sTNFRs in TMJ synovial fluid are higher in the presence of painful ADDwoR/OA, which could modulate intracapsular inflammation.
AB - The objective of this study was to detect soluble-form tumour necrosis factor receptors (sTNFRs) in temporomandibular joint (TMJ) synovial fluid aspirates, and to compare the sTNFR concentrations between painful anterior disc displacement without reduction and osteoarthritis (ADDwoR/OA) and asymptomatic TMJs. Synovial fluid was sampled from the superior TMJ cavity of 11 painful ADDwoR/OA cases (mean age: 36.9 years) and 10 asymptomatic females (mean age: 24.7 years) by diluted aspiration. The concentrations of sTNFR-I and -II in the synovial fluid were measured using human sTNFR-I and -II enzyme-linked immunosorbent assays. The total protein concentrations in synovial fluids were measured using a bicinchoninic acid protein assay kit. All data were normalised to the total protein concentration of each sample. Two-way factorial analysis of variance and post hoc multiple comparison revealed that: (1) mean normalised sTNFR-I and -II concentrations were higher in TMJ synovial aspirates from ADDwoR/OA patients than from healthy controls; (2) in the ADDwoR/OA patients and the healthy controls, the sTNFR-I concentration in TMJ synovial aspirates was higher than the sTNFR-II concentration; and (3) high TMJ synovial aspirate sTNFR-II seemed to be associated with less TMJ pain and a less restricted range of mouth opening in the ADDwoR/OA patients. The concentrations of sTNFRs in TMJ synovial fluid are higher in the presence of painful ADDwoR/OA, which could modulate intracapsular inflammation.
KW - Osteoarthritis (OA)
KW - Soluble-form receptor
KW - Synovial fluid
KW - Temporomandibular joint (TMJ)
KW - TNFα
UR - http://www.scopus.com/inward/record.url?scp=0742288637&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0742288637&partnerID=8YFLogxK
U2 - 10.1016/j.archoralbio.2003.07.001
DO - 10.1016/j.archoralbio.2003.07.001
M3 - Article
VL - 49
SP - 133
EP - 142
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 2
ER -