Solubilization and reconstitution of a lysosomal H1-pump

Yoshinori Moriyama, Tatsuya Takano, Shoji Ohkuma

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

H+-ATPase was solubilized from the lysosomal membranes of rat liver with lysophosphatidylcholine. The detergent was removed with Biobeads SM2, and the proteoliposomes were prepared by rapid dilution of a mixture of the solubilized ATPase, octyl-β-D-glucopyranoside and liposomes. The proteoliposomes exhibited ATP-dependent fluorescence quenching of quinacrine and bis(3-phenyl-5-oxoisox-asol-4-yl)pentamethine oxonol. These results suggested that the electrogenic lysosomal H+-pump was solubilized and reconstituted into proteoliposomes.

Original languageEnglish
Pages (from-to)927-930
Number of pages4
JournalJournal of biochemistry
Volume96
Issue number3
DOIs
Publication statusPublished - Jan 1 1984

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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