Solubilization and reconstitution of a lysosomal H1-pump

Yoshinori Moriyama, Tatsuya Takano, Shoji Ohkuma

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

H+-ATPase was solubilized from the lysosomal membranes of rat liver with lysophosphatidylcholine. The detergent was removed with Biobeads SM2, and the proteoliposomes were prepared by rapid dilution of a mixture of the solubilized ATPase, octyl-β-D-glucopyranoside and liposomes. The proteoliposomes exhibited ATP-dependent fluorescence quenching of quinacrine and bis(3-phenyl-5-oxoisox-asol-4-yl)pentamethine oxonol. These results suggested that the electrogenic lysosomal H+-pump was solubilized and reconstituted into proteoliposomes.

Original languageEnglish
Pages (from-to)927-930
Number of pages4
JournalJournal of Biochemistry
Volume96
Issue number3
Publication statusPublished - 1984
Externally publishedYes

Fingerprint

ATP Synthase
Pump
Pumps
Liposomes
Adenosinetriphosphate
Detergents
Quenching
Liver
Fluorescence
Dilution
Rats
Proton Pumps
Quinacrine
Lysophosphatidylcholines
Proton-Translocating ATPases
Membrane
Membranes
Adenosine Triphosphatases
Dependent
Adenosine Triphosphate

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Moriyama, Y., Takano, T., & Ohkuma, S. (1984). Solubilization and reconstitution of a lysosomal H1-pump. Journal of Biochemistry, 96(3), 927-930.

Solubilization and reconstitution of a lysosomal H1-pump. / Moriyama, Yoshinori; Takano, Tatsuya; Ohkuma, Shoji.

In: Journal of Biochemistry, Vol. 96, No. 3, 1984, p. 927-930.

Research output: Contribution to journalArticle

Moriyama, Y, Takano, T & Ohkuma, S 1984, 'Solubilization and reconstitution of a lysosomal H1-pump', Journal of Biochemistry, vol. 96, no. 3, pp. 927-930.
Moriyama Y, Takano T, Ohkuma S. Solubilization and reconstitution of a lysosomal H1-pump. Journal of Biochemistry. 1984;96(3):927-930.
Moriyama, Yoshinori ; Takano, Tatsuya ; Ohkuma, Shoji. / Solubilization and reconstitution of a lysosomal H1-pump. In: Journal of Biochemistry. 1984 ; Vol. 96, No. 3. pp. 927-930.
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