Solubilization and reconstitution of a lysosomal H1-pump

Yoshinori Moriyama, Tatsuya Takano, Shoji Ohkuma

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Abstract

H+-ATPase was solubilized from the lysosomal membranes of rat liver with lysophosphatidylcholine. The detergent was removed with Biobeads SM2, and the proteoliposomes were prepared by rapid dilution of a mixture of the solubilized ATPase, octyl-β-D-glucopyranoside and liposomes. The proteoliposomes exhibited ATP-dependent fluorescence quenching of quinacrine and bis(3-phenyl-5-oxoisox-asol-4-yl)pentamethine oxonol. These results suggested that the electrogenic lysosomal H+-pump was solubilized and reconstituted into proteoliposomes.

Original languageEnglish
Pages (from-to)927-930
Number of pages4
JournalJournal of Biochemistry
Volume96
Issue number3
Publication statusPublished - 1984
Externally publishedYes

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ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Moriyama, Y., Takano, T., & Ohkuma, S. (1984). Solubilization and reconstitution of a lysosomal H1-pump. Journal of Biochemistry, 96(3), 927-930.