Solubilization and chemical characterization of an insoluble matrix protein in the gastroliths of a crayfish, procambarus clarkii

Katsuaki Ishii, Naoaki Tsutsui, Toshiki Watanabe, Tadashi Yanagisawa, Hiromichi Nagasawa

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The gastrolith of the crayfish Procambarus clarkii contains a small amount of an organic matrix that is mainly chitin and proteins, together with a large amount of calcium carbonate. As the first step to understand the mechanism of calcification, we tried to characterize matrix proteins in the gastrolith. An insoluble matrix protein, referred to as gastrolith matrix protein, was made soluble with 1% SDS containing 10 mM dithiothreitol, and was purified by reverse-phase high-performance liquid chromatography. The protein had a molecular weight of about 50,500 and a blocked amino terminus. By enzymatic digestion and microsequencing, five partial amino acid sequences with a total of 225 amino acid residues were identified and found to include a repetitive sequence not reported previously.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number2
DOIs
Publication statusPublished - 1998

Keywords

  • Calcification
  • Crayfish
  • Gastrolith
  • Matrix protein
  • Procambarus clarkii

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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