Solubilization and Chemical Characterization of an Insoluble Matrix Protein in the Gastroliths of a Crayfish, Procambarus clarkii

Katsuaki Ishii, Naoaki Tsutsui, Toshiki Watanabe, Tadashi Yanagisawa, Hiromichi Nagasawa

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The gastrolith of the crayfish Procambarus clarkii contains a small amount of an organic matrix that is mainly chitin and proteins, together with a large amount of calcium carbonate. As the first step to understand the mechanism of calcification, we tried to characterize matrix proteins in the gastrolith. An insoluble matrix protein, referred to as gastrolith matrix protein, was made soluble with 1% SDS containing 10 mM dithiothreitol, and was purified by reverse-phase high-performance liquid chromatography. The protein had a molecular weight of about 50,500 and a blocked amino terminus. By enzymatic digestion and microsequencing, five partial amino acid sequences with a total of 225 amino acid residues were identified and found to include a repetitive sequence not reported previously.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number2
Publication statusPublished - Feb 1998
Externally publishedYes

Fingerprint

Astacoidea
Procambarus clarkii
crayfish
solubilization
Proteins
proteins
Amino acids
Amino Acids
Chitin
repetitive sequences
dithiothreitol
Calcium Carbonate
reversed-phase liquid chromatography
Nucleic Acid Repetitive Sequences
Dithiothreitol
High performance liquid chromatography
Calcium carbonate
calcification
Reverse-Phase Chromatography
calcium carbonate

Keywords

  • Calcification
  • Crayfish
  • Gastrolith
  • Matrix protein
  • Procambarus clarkii

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Solubilization and Chemical Characterization of an Insoluble Matrix Protein in the Gastroliths of a Crayfish, Procambarus clarkii. / Ishii, Katsuaki; Tsutsui, Naoaki; Watanabe, Toshiki; Yanagisawa, Tadashi; Nagasawa, Hiromichi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 62, No. 2, 02.1998, p. 291-296.

Research output: Contribution to journalArticle

Ishii, Katsuaki ; Tsutsui, Naoaki ; Watanabe, Toshiki ; Yanagisawa, Tadashi ; Nagasawa, Hiromichi. / Solubilization and Chemical Characterization of an Insoluble Matrix Protein in the Gastroliths of a Crayfish, Procambarus clarkii. In: Bioscience, Biotechnology and Biochemistry. 1998 ; Vol. 62, No. 2. pp. 291-296.
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