Solubilization and characterization of the acceptor for Clostridium botulinum type B neutroxin from rat brain synaptic membranes

Tei-ichi Nishiki, Jun Ogasawara, Yoichi Kamata, Shunji Kozaki

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The acceptor for Clostridium botulinum type B neurotoxin was solubilized from rat brain synaptic membrane with nonionic detergent, nonanoyl-N-methylglucamide (MEGA-9). The solubilized acceptor was assayed for the binding activity by precipitating the acceptor with acetone in the presence of phosphatidylcholine. 125Ilabeled neutroxin specifically bound to the lipid vesicles having incorporated the acceptor together with gangliosided. The lipid vesicles having incorporated either the acceptor or gangliosides alone showed extremely low binding activity. The treatment of the solubilized acceptor with lysyl endopeptidase and accompanying an N-linked carbohydrate moiety. The observations suggest also that a protein acceptor/ganglioside complex may be required to form the functional toxin receptor.

Original languageEnglish
Pages (from-to)333-338
Number of pages6
JournalBiochimica et Biophysica Acta - General Subjects
Volume1158
Issue number3
DOIs
Publication statusPublished - Nov 28 1993
Externally publishedYes

Fingerprint

Clostridium botulinum type B
Synaptic Membranes
Clostridium
lysyl endopeptidase
Gangliosides
Rats
Brain
Membranes
Lipids
Neurotoxins
Acetone
Phosphatidylcholines
Detergents
Carbohydrates
Proteins
nonanoyl-N-methylglucamide

Keywords

  • (C. botulinum)
  • Ganglioside
  • Neurotoxin
  • Protein acceptor
  • Solubilization
  • Synaptic membrane
  • Toxin binding

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Solubilization and characterization of the acceptor for Clostridium botulinum type B neutroxin from rat brain synaptic membranes. / Nishiki, Tei-ichi; Ogasawara, Jun; Kamata, Yoichi; Kozaki, Shunji.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1158, No. 3, 28.11.1993, p. 333-338.

Research output: Contribution to journalArticle

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