Sol-gel preparation of HAp-coated silica macrospheres from water glass and their protein adsorption

Jie Li, Yuki Shirosaki, Satoshi Hayakawa, Artemis Stamboulis, Akiyoshi Osaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Silica gel macrospheres of 2~4 mm in size, and wrapped with Ca-alginate chelate film were prepared as the substrate for hydroxyapatite coating layer, using water glass as the silica source. Those Ca-SiO2 macrospheres were soaked in a 1:1 (volume) mixture of ethanol and 0.1 M Na 2HPO4 to deposit hydroxyapatite layer (HAp-SiO2 macrospheres). Adsorption of bovine serum albumin and egg lysozyme on those Ca-SiO2 and HAp-SiO2 macrospheres under physiological pH (7.2) was well correlated to the Langmuir-type adsorption equation. The electrostatic interactions between the protein molecules and those macrospheres well interpret the adsorption isotherms, while the mesopores in the Ca-SiO 2contributed to some extent. A multi-layer adsorption model was proposed.

Original languageEnglish
Title of host publicationKey Engineering Materials
Pages637-640
Number of pages4
Volume529-530
Edition1
DOIs
Publication statusPublished - 2013
Event24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012 - Fukuoka, Japan
Duration: Oct 21 2012Oct 24 2012

Publication series

NameKey Engineering Materials
Number1
Volume529-530
ISSN (Print)10139826

Other

Other24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012
CountryJapan
CityFukuoka
Period10/21/1210/24/12

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Keywords

  • Hydroxyapatite coating
  • Protein adsorption
  • Silica gel macrosphere
  • Water glass

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Li, J., Shirosaki, Y., Hayakawa, S., Stamboulis, A., & Osaka, A. (2013). Sol-gel preparation of HAp-coated silica macrospheres from water glass and their protein adsorption. In Key Engineering Materials (1 ed., Vol. 529-530, pp. 637-640). (Key Engineering Materials; Vol. 529-530, No. 1). https://doi.org/10.4028/www.scientific.net/KEM.529-530.637