Sol-gel preparation of apatite-coated silica macrospheres from water glass and their adsorption of bovine serum albumin and lysozyme

Jie Li, Yuki Shirosaki, Satoshi Hayakawa, Artemis Stamboulis, Akiyoshi Osaka

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Silica gel macrospheres were prepared from water glass as the silica source. The water glass solution was mixed with sodium alginate solution and added dropwisely to 1M CaCl2 solution to form macrospheres with 34 and 2.6mm in diameter. Those SiO2 macrospheres were soaked in 1:1 (volume) mixture of ethanol and 0.1M Na2HPO4 to deposit hydroxyapatite layer (HAp-SiO2 macrospheres). Bovine serum albumin (BSA) and egg lysozyme (LYZ) were adsorbed those SiO2 and HAp-SiO2 macrospheres. The amount of proteins adsorbed was well correlated to the Langmuir-type adsorption equation. The electrostatic interaction was predominant between the macrosphere surface and the protein molecules. Protein molecular entrapment in the mesopores (5-25 nm) was also probable for the SiO2 macrospheres but not applicable to the HAp-SiO2 ones without the mesopores. From consideration that protein molecule has a few groups with negative or positive partial charges, a multi-layer adsorption model was proposed.

Original languageEnglish
Pages (from-to)355-361
Number of pages7
JournalJournal of the Ceramic Society of Japan
Volume120
Issue number1405
DOIs
Publication statusPublished - Sep 2012

Keywords

  • Apatite deposition
  • Microstructure
  • Protein adsorption
  • Silica macrospheres
  • Sol-gel processing

ASJC Scopus subject areas

  • Ceramics and Composites
  • Chemistry(all)
  • Condensed Matter Physics
  • Materials Chemistry

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