Sodium-level-sensitive sodium channel Nax is expressed in glial laminate processes in the sensory circumventricular organs

Eiji Watanabe, Takeshi Y. Hiyama, Hidetada Shimizu, Ryuji Kodama, Noriko Hayashi, Seiji Miyata, Yuchio Yanagawa, Kunihiko Obata, Masaharu Noda

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Nax is an atypical sodium channel that is assumed to be a descendant of the voltage-gated sodium channel family. Our recent studies on the Nax-gene-targeting mouse revealed that Nax channel is localized to the circumventricular organs (CVOs), the central loci for the salt and water homeostasis in mammals, where the Nax channel serves as a sodium-level sensor of the body fluid. To understand the cellular mechanism by which the information sensed by Nax channels is transferred to the activity of the organs, we dissected the subcellular localization of Na x in the present study. Double-immunostaining and immunoelectron microscopic analyses revealed that Nax is exclusively localized to perineuronal lamellate processes extended from ependymal cells and astrocytes in the organs. In addition, glial cells isolated from the subfornical organ, one of the CVOs, were sensitive to an increase in the extracellular sodium level, as analyzed by an ion-imaging method. These results suggest that glial cells bearing the Nax channel are the first to sense a physiological increase in the level of sodium in the body fluid, and they regulate the neural activity of the CVOs by enveloping neurons. Close communication between inexcitable glial cells and excitable neural cells thus appears to be the basis of the central control of the salt homeostasis.

Original languageEnglish
Pages (from-to)R568-R576
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume290
Issue number3
DOIs
Publication statusPublished - Mar 1 2006
Externally publishedYes

Fingerprint

Sodium Channels
Neuroglia
Sodium
Body Fluids
Homeostasis
Salts
Subfornical Organ
Voltage-Gated Sodium Channels
Gene Targeting
Astrocytes
Mammals
Communication
Ions
Neurons
Water
Circumventricular Organs

Keywords

  • Astrocyte
  • Ependymal cell
  • GABAergic neuron
  • Glial sodium channel
  • Na2
  • Neuron-glia interaction
  • Salt homeostasis
  • Sodium sensor

ASJC Scopus subject areas

  • Physiology

Cite this

Sodium-level-sensitive sodium channel Nax is expressed in glial laminate processes in the sensory circumventricular organs. / Watanabe, Eiji; Hiyama, Takeshi Y.; Shimizu, Hidetada; Kodama, Ryuji; Hayashi, Noriko; Miyata, Seiji; Yanagawa, Yuchio; Obata, Kunihiko; Noda, Masaharu.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 290, No. 3, 01.03.2006, p. R568-R576.

Research output: Contribution to journalArticle

Watanabe, Eiji ; Hiyama, Takeshi Y. ; Shimizu, Hidetada ; Kodama, Ryuji ; Hayashi, Noriko ; Miyata, Seiji ; Yanagawa, Yuchio ; Obata, Kunihiko ; Noda, Masaharu. / Sodium-level-sensitive sodium channel Nax is expressed in glial laminate processes in the sensory circumventricular organs. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2006 ; Vol. 290, No. 3. pp. R568-R576.
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