SNARE Protein Recycling by αSNAP and βSNAP Supports Synaptic Vesicle Priming

Andrea Burgalossi, Sangyong Jung, Guido Meyer, Wolf J. Jockusch, Olaf Jahn, Holger Taschenberger, Vincent M. O'Connor, Tei-ichi Nishiki, Masami Takahashi, Nils Brose, Jeong Seop Rhee

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Neurotransmitter release proceeds by Ca2+-triggered, SNARE-complex-dependent synaptic vesicle fusion. After fusion, the ATPase NSF and its cofactors α- and βSNAP disassemble SNARE complexes, thereby recycling individual SNAREs for subsequent fusion reactions. We examined the effects of genetic perturbation of α- and βSNAP expression on synaptic vesicle exocytosis, employing a new Ca2+ uncaging protocol to study synaptic vesicle trafficking, priming, and fusion in small glutamatergic synapses of hippocampal neurons. By characterizing this protocol, we show that synchronous and asynchronous transmitter release involve different Ca2+ sensors and are not caused by distinct releasable vesicle pools, and that tonic transmitter release is due to ongoing priming and fusion of new synaptic vesicles during high synaptic activity. Our analysis of α- and βSNAP deletion mutant neurons shows that the two NSF cofactors support synaptic vesicle priming by determining the availability of free SNARE components, particularly during phases of high synaptic activity.

Original languageEnglish
Pages (from-to)473-487
Number of pages15
JournalNeuron
Volume68
Issue number3
DOIs
Publication statusPublished - Nov 4 2010

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SNARE Proteins
Synaptic Vesicles
Recycling
N-Ethylmaleimide-Sensitive Proteins
Neurons
Exocytosis
Synapses
Neurotransmitter Agents

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Burgalossi, A., Jung, S., Meyer, G., Jockusch, W. J., Jahn, O., Taschenberger, H., ... Rhee, J. S. (2010). SNARE Protein Recycling by αSNAP and βSNAP Supports Synaptic Vesicle Priming. Neuron, 68(3), 473-487. https://doi.org/10.1016/j.neuron.2010.09.019

SNARE Protein Recycling by αSNAP and βSNAP Supports Synaptic Vesicle Priming. / Burgalossi, Andrea; Jung, Sangyong; Meyer, Guido; Jockusch, Wolf J.; Jahn, Olaf; Taschenberger, Holger; O'Connor, Vincent M.; Nishiki, Tei-ichi; Takahashi, Masami; Brose, Nils; Rhee, Jeong Seop.

In: Neuron, Vol. 68, No. 3, 04.11.2010, p. 473-487.

Research output: Contribution to journalArticle

Burgalossi, A, Jung, S, Meyer, G, Jockusch, WJ, Jahn, O, Taschenberger, H, O'Connor, VM, Nishiki, T, Takahashi, M, Brose, N & Rhee, JS 2010, 'SNARE Protein Recycling by αSNAP and βSNAP Supports Synaptic Vesicle Priming', Neuron, vol. 68, no. 3, pp. 473-487. https://doi.org/10.1016/j.neuron.2010.09.019
Burgalossi A, Jung S, Meyer G, Jockusch WJ, Jahn O, Taschenberger H et al. SNARE Protein Recycling by αSNAP and βSNAP Supports Synaptic Vesicle Priming. Neuron. 2010 Nov 4;68(3):473-487. https://doi.org/10.1016/j.neuron.2010.09.019
Burgalossi, Andrea ; Jung, Sangyong ; Meyer, Guido ; Jockusch, Wolf J. ; Jahn, Olaf ; Taschenberger, Holger ; O'Connor, Vincent M. ; Nishiki, Tei-ichi ; Takahashi, Masami ; Brose, Nils ; Rhee, Jeong Seop. / SNARE Protein Recycling by αSNAP and βSNAP Supports Synaptic Vesicle Priming. In: Neuron. 2010 ; Vol. 68, No. 3. pp. 473-487.
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