SmdAB, a heterodimeric ABC-type multidrug efflux pump, in Serratia marcescens

Taira Matsuo, Jing Chen, Yusuke Minato, Wakano Ogawa, Tohru Mizushima, Teruo Kuroda, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We cloned genes, designated smdAB, that encode a multidrug efflux pump from the chromosomal DNA of clinically isolated Serratia marcescens NUSM8906. For cells of the drug-hypersensitive strain Escherichia coli KAM32 harboring a recombinant plasmid carrying smdAB, structurally unrelated antimicrobial agents such as norfloxacin, tetracycline, 4′,6-diamidino-2-phenylindole (DAPI), and Hoechst 33342 showed elevated MICs. The deduced amino acid sequences of both SmdA and SmdB exhibited similarities to the sequences of ATP-binding cassette (ABC)-type multidrug efflux pumps. The efflux of DAPI and Hoechst 33342 from E. coli cells expressing SmdAB was observed, and the efflux activities were inhibited by sodium o-vanadate, which is a well-known ATPase inhibitor. The introduction of smdA or smdB alone into E. coli KAM32 did not elevate the MIC of DAPI; thus, both SmdA and SmdB were required for function. These results indicate that SmdAB is probably a heterodimeric multidrug efflux pump of the ABC family in S. marcescens.

Original languageEnglish
Pages (from-to)648-654
Number of pages7
JournalJournal of Bacteriology
Volume190
Issue number2
DOIs
Publication statusPublished - Jan 2008

Fingerprint

Serratia marcescens
Adenosine Triphosphate
Escherichia coli
Norfloxacin
Vanadates
Anti-Infective Agents
Tetracycline
Adenosine Triphosphatases
Amino Acid Sequence
Plasmids
DNA
Pharmaceutical Preparations
Genes
2-phenylindole
HOE 33342

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Matsuo, T., Chen, J., Minato, Y., Ogawa, W., Mizushima, T., Kuroda, T., & Tsuchiya, T. (2008). SmdAB, a heterodimeric ABC-type multidrug efflux pump, in Serratia marcescens. Journal of Bacteriology, 190(2), 648-654. https://doi.org/10.1128/JB.01513-07

SmdAB, a heterodimeric ABC-type multidrug efflux pump, in Serratia marcescens. / Matsuo, Taira; Chen, Jing; Minato, Yusuke; Ogawa, Wakano; Mizushima, Tohru; Kuroda, Teruo; Tsuchiya, Tomofusa.

In: Journal of Bacteriology, Vol. 190, No. 2, 01.2008, p. 648-654.

Research output: Contribution to journalArticle

Matsuo, T, Chen, J, Minato, Y, Ogawa, W, Mizushima, T, Kuroda, T & Tsuchiya, T 2008, 'SmdAB, a heterodimeric ABC-type multidrug efflux pump, in Serratia marcescens', Journal of Bacteriology, vol. 190, no. 2, pp. 648-654. https://doi.org/10.1128/JB.01513-07
Matsuo T, Chen J, Minato Y, Ogawa W, Mizushima T, Kuroda T et al. SmdAB, a heterodimeric ABC-type multidrug efflux pump, in Serratia marcescens. Journal of Bacteriology. 2008 Jan;190(2):648-654. https://doi.org/10.1128/JB.01513-07
Matsuo, Taira ; Chen, Jing ; Minato, Yusuke ; Ogawa, Wakano ; Mizushima, Tohru ; Kuroda, Teruo ; Tsuchiya, Tomofusa. / SmdAB, a heterodimeric ABC-type multidrug efflux pump, in Serratia marcescens. In: Journal of Bacteriology. 2008 ; Vol. 190, No. 2. pp. 648-654.
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