Small interfering RNA targeting CD81 decreased the expression of tumor necrosis factor-alpha and synoviolin in SW982 cells

Takae Hanada, Erika Fujimoto, Hiroki Mori, Toshitaka Oohashi, Yoshifumi Ninomiya, Tohru Nakanishi

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5 Citations (Scopus)

Abstract

Rheumatoid arthritis (RA), one of the most common articular diseases, is characterized by chronic inflammation, hyperproliferation of synovial cells, and bone destruction. CD81, which belongs to a family of cell-surface proteins (tetraspanin), is known to be up-regulated in RA synoviocytes. Recently, we showed the therapeutic effect of using small interfering RNA in targeting CD81 (CD81 siRNA) for RA. Here we show how tumor necrosis factor-alpha (TNF-α) can induce the expression of synoviolin, which is decreased by the addition of CD81 siRNA in SW982 cells. In addition, CD81 siRNA decreases the expression of TNF-α in SW982 cells, suggesting that TNF-α stimulates the expression of synoviolin via CD81. Furthermore, knock down of CD81 by the addition of CD81 siRNA decreases the expression of TNF-α, and TNF-α-induces expression of synoviolin in direct and indirect ways. These results in SW982 cells might explain the therapeutic effect of CD81 siRNA in animal models.

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalJournal of Hard Tissue Biology
Volume22
Issue number2
DOIs
Publication statusPublished - 2013

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Keywords

  • CD81
  • Rheumatoid arthritis
  • SiRNA
  • SW982 cell
  • Synoviolin
  • Tumor necrosis factor-alpha

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

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