Small cell lung cancer can express CD34 antigen

Hiromichi Yamane, Katsuyuki Kiura, Masahiro Tabata, Akihiro Bessho, Tadashi Tsuchida, Kinya Motoda, Akio Hiraki, Hiroshi Ueoka, Mine Harada

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In humans CD34 is a valid and reliable marker for hematopoletic stem and progenitor cells. In general, solid tumors, with the exception of endothelial cancers, do not express CD34. Accordingly, immunological selection of CD34+ hematopoietic stem/progenitor cells can be used to remove CD34- malignant cells in the setting of autotransplantation. To rule out CD34 expression on tumor cells from small cell lung cancer (SCLC), eleven SCLC cell lines were analyzed by flow cytometry. Interestingy, two of these were positive for CD34 and their expression of full-length CD34 was further confirmed by reverse transcriptase and polymerase chain reaction (RT-PCR). This finding indicates that prior to subjecting SCLC patients to the above treatment modality, preparing CD34+ hematopoietic stem/progenitor cells from SCLC patients for autotransplantation, CD34 expression on their tumour cells should be screened using immunohistochemistry and/or flow cytometry.

Original languageEnglish
Pages (from-to)3627-3632
Number of pages6
JournalAnticancer research
Volume17
Issue number5 A
Publication statusPublished - Sep 1 1997

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Keywords

  • CD34
  • Flow cytometry
  • RT-PCR
  • Small cell lung cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Yamane, H., Kiura, K., Tabata, M., Bessho, A., Tsuchida, T., Motoda, K., Hiraki, A., Ueoka, H., & Harada, M. (1997). Small cell lung cancer can express CD34 antigen. Anticancer research, 17(5 A), 3627-3632.